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首页> 外文期刊>Sensors and Actuators. B, Chemical >An improved Severinghaus-type carbon dioxide microelectrode for use in biofilms
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An improved Severinghaus-type carbon dioxide microelectrode for use in biofilms

机译：用于生物膜的改进的Severinghaus型二氧化碳微电极

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摘要

Severinghaus-type carbon dioxide microelectrodes with a tip diameter of less than 20 μm were constructed using anodically grown iridium oxide film (AIROF) as the internal pH sensor. The AIROF, which was formed at the tip of the iridium wire by cyclic voltammetry in diluted sulfuric acid, showed a super-Nernstian response to pH changes, with a slope between 65 and 80 mV/pH. Therefore, our microelectrodes were more sensitive to changes in carbon dioxide concentration than the previously described microelectrodes, which used liquid ion exchanger (LIX) pH membranes and had a Nernstian response of 59 mV/pH. When calibrated, our microelectrodes showed 65-76 mV per decade of μatm pCO_2, and were more sensitive than the microelectrodes using LIX membranes, which showed 57 mV per decade of μatm pCO_2. AIROF electrodes were serviceable after 1 month of storage, showing only a modest potential drift of 0.03 +- 0.01 mV/h. Their utility was demonstrated by measuring CO_2 concentration profiles in Staphylococcus aureus (MN8) biofilms.
机译：使用阳极生长的氧化铱膜（AIROF）作为内部pH传感器，构建尖端直径小于20μm的Severinghaus型二氧化碳微电极。 AIROF是在稀硫酸中通过循环伏安法在铱丝的尖端形成的，对pH的变化表现出超能斯特响应，斜率在65至80 mV / pH之间。因此，与使用液体离子交换器（LIX）pH膜并具有59 mV / pH的Nernstian响应的前述微电极相比，我们的微电极对二氧化碳浓度的变化更敏感。校准后，我们​​的微电极每10 µm pCO_2的微电极显示65-76 mV，比使用LIX膜每10 µm pCO_2的57 mV的微电极更敏感。 AIROF电极在储存1个月后即可使用，仅显示出0.03±0.01 mV / h的适度电位漂移。通过测量金黄色葡萄球菌（MN8）生物膜中的CO_2浓度曲线证明了其实用性。

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