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首页> 外文期刊>Sensors Journal, IEEE >All Solid-State Potentiometric Biosensors for Creatinine Determination Based on pH and Ammonium Electrodes
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All Solid-State Potentiometric Biosensors for Creatinine Determination Based on pH and Ammonium Electrodes

机译：基于pH和铵电极的所有用于肌酐测定的固态电位生物传感器

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This work investigates a disposable creatinine potentiometric biosensor based on a pH-sensitive electrode and an ammonium ion-selective electrode. An enzyme, creatinine iminohydrolase (CIH) was immobilized directly onto the surface of the electrodes by the entrapment method. According to the experimental results, the creatinine biosensors based on the pH-sensitive electrode and ammonium ion-selective electrode show a rapid response time ( ${rm t} _{95} ≪ 30~$s) and a linear dynamic response range for creatinine determination between 40 $mu$M and 140 $mu$M. Furthermore, the ammonium ion-selective electrode-based biosensors exhibited good operational stability (over 14 times) and storage stability (over 40 days). By discussing the reproducibility and pH effects of the creatinine biosensors, features of creatinine biosensors based on ammonium ion-selective electrodes are described. Moreover, development of a low-cost, solid-state, disposable potentiometric creatinine biosensor based on pH-sensitive electrodes and ammonium ion-selective electrodes is discussed.
机译：这项工作研究了基于pH敏感电极和铵离子选择电极的一次性肌酐电位生物传感器。通过包埋法将肌酸酐亚氨基水解酶（CIH）直接固定在电极表面上。根据实验结果，基于pH敏感电极和铵离子选择电极的肌酐生物传感器显示快速响应时间（$ {rm t} _ {95}≪ 30〜$ s）和线性动态响应范围。肌酐测定介于40μM和140μM之间。此外，基于铵离子选择电极的生物传感器表现出良好的操作稳定性（超过14倍）和存储稳定性（超过40天）。通过讨论肌酸酐生物传感器的重现性和pH效应，描述了基于铵离子选择电极的肌酸酐生物传感器的特征。此外，讨论了基于pH敏感电极和铵离子选择性电极的低成本，固态，一次性电位肌酐生物传感器的开发。

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