...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>IEEE sensors journal >An Integrated Whole-Cell Detection Platform for Heavy Metal Ions
【24h】

An Integrated Whole-Cell Detection Platform for Heavy Metal Ions

机译：重金属离子的集成全细胞检测平台

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Heavy metal ions, such as Pb2+ and Cd2+, are main sources of water quality contaminants. Whole-cell detection has recently been actively researched to use genetically modified bacteria to sense the presence of heavy metal ions in water or soil. Compared to common cell-free methods, such as immunosensor and electrochemical sensor, whole-cell sensors need simple sample preparation and can continuously sense metal contaminants in the environment with the cell culture. Nonetheless, whole-cell sensors so far have demonstrated sensitivity too low for water quality monitoring. In this work, we developed an integrated platform based on whole-cell detection. Our genetically-modified E. coli bacteria has a good specificity on lead and cadmium. The platform integrates the light source, cultured cells and the detector for the first time. The integrated platform shows good sensitivity. Limits of detection for Pb2+ and Cd2+ are 518 ppb and 44.8 ppb respectively.
机译：重金属离子，如PB2 +和CD2 +，是水质污染物的主要来源。最近已经积极研究了全细胞检测，以使用转基因细菌来感测水或土壤中重金属离子的存在。与免疫传感器和电化学传感器的常见无细胞方法相比，全细胞传感器需要简单的样品制备，并且可以在细胞培养中连续感知环境中的金属污染物。尽管如此，到目前为止，整个细胞传感器已经证明了水质监测的敏感性太低。在这项工作中，我们根据全单元检测开发了一个集成平台。我们的遗传修饰的大肠杆菌细菌对铅和镉具有良好的特异性。该平台首次集成光源，培养的细胞和检测器。集成平台显示出良好的敏感性。 PB2 +和CD2 +的检测限差异分别为518ppb和44.8 ppb。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号