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首页> 外文期刊>IEEE sensors journal >Wireless Portable Evaluation Platform for Photodynamic Therapy: In vitro Assays on Human Gastric Adenocarcinoma Cells
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Wireless Portable Evaluation Platform for Photodynamic Therapy: In vitro Assays on Human Gastric Adenocarcinoma Cells

机译：用于光动力疗法的无线便携式评估平台：人胃腺癌细胞体外测定

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Photodynamic Therapy (PDT) is a technique used in the selective destruction of cancer cells and requires the previous knowledge of light dosimetry and photosensitizer drug (PS) concentration to apply on cells. Therefore, a laboratory platform is required for further evaluation, e.g. to make evaluation and assays on previously collected cells by biopsy to determine the best combination of light dose and PS concentration. In this context, this paper presents a low-cost, low-sized and flexible Wireless Portable Evaluation Platform for PDT assays on cells. The W-PEP was tested and evaluated using assays with Human Gastric Adenocarcinoma (AGS) cells. The AGS cells were exposed to the 5-aminolevulinic acid (ALA), which is a precursor of the photosensitive agent Protoporphyrin IX (PpIX) with absorption at 635nm. The W-PEP is composed by 16 small-sized LEDs matrix with peak transmittance at 634nm mounted in a PCB and controlled by ESP32-DevKitC(R), a power bank to supply the lighting and the controlling systems and 3D printed components specially designed to allow portability and PDT assays. A graphical user interface (GUI) application for mobile devices was developed allowing the bi-directional communication between the smartphone and W-PEP via Bluetooth. The measurements with AGS cells proved this W-PEP was effective in its purpose and promoted cell death in samples treated with ALA and a final light dose of 5J/cm(2) after 49 minutes of light exposure. This W-PEP has 140mm of both length and width, 75mm of height and costs about $82.10. To finish, this system checks the optimal conditions that will be applied in the treatment after, thereby decreasing the costs and time of the application.
机译：光动力治疗（PDT）是一种用于癌细胞选择性破坏的技术，并且需要先前的光剂量和光敏剂药物（PS）浓度施加在细胞上。因此，进一步评估需要实验室平台，例如，通过活组织检查在预先收集的细胞上进行评估和测定以确定光剂量和PS浓度的最佳组合。在这种情况下，本文介绍了用于电池的PDT测定的低成本，低尺寸和灵活的无线便携式评估平台。使用具有人胃腺癌（AGS）细胞的测定来测试和评估W-PEP。将AGS细胞暴露于5-氨基乙酰丙烯酸（ALA），其是感光剂原子蛋白IX（PPIX）的前体，在635nm处吸收。 W-PEP由16个小尺寸LED矩阵组成，峰值透射率在PCB中安装在634nm，并由ESP32-Devkitc（R）控制，该电源组供电，以提供专门设计的照明和控制系统和3D印刷部件。允许可移植性和PDT测定。开发了一种用于移动设备的图形用户界面（GUI）应用程序，允许通过蓝牙进行智能手机和W-PEP之间的双向通信。通过AGS细胞的测量结果证明了该W-PEP在其目的中有效，并在光暴露49分钟后促进用ALA处理的样品和最终光剂量的5J / cm（2）的细胞死亡。这款W-PEP的长度和宽度为140毫米，高度为75毫米，费用约为82.10美元。为了完成，该系统检查后检查的最佳条件，从而降低申请的成本和时间。

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