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首页> 外文期刊>Soil Science Society of America Journal >Nitrogen-15 and Oxygen-18 Natural Abundance of Potassium Chloride Extractable Soil Nitrate Using the Denitrifier Method
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Nitrogen-15 and Oxygen-18 Natural Abundance of Potassium Chloride Extractable Soil Nitrate Using the Denitrifier Method

机译：反硝化法提取氯化钾可硝化土壤中氮15和氧18的自然丰度

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In agroecosystems, most isotopic investigations of NO3– involve the use of tracers that are artificially enriched in 15N. Although the dual isotope composition of NO3–— 15N and 18O is especially beneficial for understanding the origin and fate of NO3–, its use for KCl-extractable soil NO3– has been hampered by the lack of a suitable analytical technique. Our objective was to test whether the denitrifier method, whereby NO3– is reduced to N2O before mass spectrometric analysis, can be used to determine the N and O isotopic composition of NO3– from 2 M KCl soil extracts. Several internationally accepted NO3–standards were dissolved in 2 M KCl, the conventional extractant for soil inorganic N, and inoculated with the bacterial strain Pseudomonas aureofaciens (ATCC no. 13985). The standard deviation of the NO3– standards analyzed did not exceed 0.2 for 15N and 0.3 for 18O values. After appropriate corrections, differences between our measured and consensus 15N and 18O values of standard NO3– generally were within the standard deviations given for the consensus values. Both 15N and 18O values were reproducible among separate analytical runs. The method was also tested on genuine 2 M KCl extracts from unfertilized and fertilized soils. Depending on N fertilization, the soils had distinct 15N and 18O values, which were attributed to amendment with NH4NO3 fertilizer. Hence, our data indicate that the denitrifier method provides a fast, reliable, precise, and accurate way of simultaneously analyzing the natural abundances of 15N and 18O in KCl-extractable soil NO3–.
机译：在农业生态系统中，大多数NO 3 – 的同位素研究都涉及使用在 15 N。尽管NO 3 – — 15 N和 18 O的双同位素组成对于理解NO 3 – 的起源 和去向，特别是对于KCl可萃取土壤NO 3 – 由于缺乏合适的分析技术而受到阻碍。 我们的目标是测试是否使用反硝化方法，从而 < / sup> NO 3 – 在质谱分析之前已还原为N 2 O， 可用于确定2 M KCl土壤提取物中 NO 3 – 的N和O同位素组成。将几种国际 认可的NO 3 – 标准溶解在2 M KCl中，这是用于土壤无机氮的常规提取剂，并用细菌菌株aureofaciens（ATCC编号 13985）接种 。分析 的NO 3 – 标准的标准偏差对于 15 N不超过0.2，对于不超过0.3 > 18 O值。经过适当的 校正后，我们的标准NO 的实测值和共识 15 N和 18 O值之间的差异3 – 通常在共识值给出的标准偏差内。在单独的分析 运行中， 15 N和 18 O值均可重现。还对未施肥和施肥的土壤中真正的2 M KCl提取物 进行了测试。根据氮肥的不同， 土壤具有不同的 15 N和 18 O值，这些值归因于 的修正。 NH 4 NO 3 肥料。因此，我们的数据表明 反硝化方法提供了一种快速，可靠，精确， 且准确的方式来同时分析的自然丰度 氯化钾可提取土壤NO 3 – 中的> 15 N和 18 O。

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    《Soil Science Society of America Journal》 |2007年第2期|355-361|共7页
  • 作者

    Luc Rock; Benjamin H. Ellert;

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    Agriculture & Agri-Food Canada, Lethbridge Research Centre, 5403 1st Ave. South, P.O. Box 3000, Lethbridge, AB, T1J 4B1, Canada;

    Agriculture & Agri-Food Canada, Lethbridge Research Centre, 5403 1st Ave. South, P.O. Box 3000, Lethbridge, AB, T1J 4B1, Canada;

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