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首页> 外文期刊>Sugar Tech >Valorization of Sugar-Ethanol Industry Waste Vinasse for Increased Second-Generation Ethanol Production Using Spathaspora passalidarum Yeast Strains
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Valorization of Sugar-Ethanol Industry Waste Vinasse for Increased Second-Generation Ethanol Production Using Spathaspora passalidarum Yeast Strains

机译：糖乙醇工业废料酒糟的增值，以提高第二代乙醇的生产能力，使用了Spathaspora passalidarum酵母菌株

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In this study, sugarcane bagasse hydrolysate supplemented with different proportions of sugar-ethanol industrial waste (vinasse) was used to optimize fermentative ethanol production using two Spathaspora passalidarum yeast strains isolated from decaying wood in Amazonian biome. The bagasse samples were pretreated chemically and enzymatically before using a binary factorial experimental design. The factorial design was carried out in two stages; in the first, a 2(4) factorial design was done to assess the effects of pretreated sugarcane bagasse hydrolysate and vinasse, ammonium sulfate concentration, yeast biomass concentration, and two different strains of yeast, S. passalidarum; in the second stage, a 2(3) factorial design was done to provide a more detailed analysis of the effects of vinasse supplementation on the fermentation processthis time, by modifying the ratio of sugarcane bagasse hydrolysate and vinasse and, more significantly, the same factorbiomass concentration. The results showed that the most significant factors for increasing bioethanol production in the 2(4) factorial design proved to be the following: inoculum and hydrolyzed bagasse supplementation with vinasse (the optimal being 75:25), followed by yeast concentration (optimal at 14% w/v cell wet weight) and then the type of strain used (S. passalidarum UFMG-HMD-14.1). The use of only hydrolyzed bagasse without vinasse (2(3) factorial design) had no significant influence on the results, nor did varying the concentration of nitrogen. This increase in ethanol production, which allows the same amount of ethanol productivity through a reduction in the carbon source, suggests it can lead to an improved final use for vinasse.
机译：在这项研究中，甘蔗渣水解物添加了不同比例的糖-乙醇工业废料（vinasse），用于优化发酵乙醇的生产，其中使用了两个从亚马逊河生物区的腐烂木材中分离出的Spathaspora passalidarum酵母菌株。在使用二元析因实验设计之前，先对蔗渣样品进行化学和酶处理。析因设计分两个阶段进行：首先，进行了2（4）因子分解设计，以评估预处理的甘蔗渣水解物和酒糟，硫酸铵的浓度，酵母生物量的浓度以及两种不同酵母菌株S. Passalidarum的影响。在第二阶段，通过修改甘蔗渣水解液和酒糟的比例，更重要的是，相同因子的生物量，进行了2（3）析因设计，从而提供了补充酒糟对发酵过程的影响的更详细分析。浓度。结果表明，在2（4）因子设计中，增加生物乙醇产量的最重要因素如下：接种物和水解的蔗渣加酒糟（最佳比例为75:25），然后是酵母浓度（最优浓度为14） ％w / v细胞湿重），然后使用所用菌株的类型（S. Passalidarum UFMG-HMD-14.1）。仅使用不带酒渣糖的水解蔗渣（2（3）析因设计）对结果没有显着影响，也没有改变氮的浓度。乙醇产量的增加，通过减少碳源，可以实现相同数量的乙醇生产率，这表明它可以改善酒糟的最终用途。

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