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Identification and Characterization of the Causal Agent of Sugarcane Root and Basal Stem Rot in South Sumatra, Indonesia

机译:印度尼西亚苏门答腊南部甘蔗根和茎基腐病病原菌的鉴定和表征

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摘要

Root and basal stem rot (RBSR) is a new serious disease affecting sugarcane plantation in Indonesia and can lead to devastating yield losses in crop-producing areas, particularly in Lampung and South Sumatra. This disease was first described in Taiwan. Based on morphological characteristics, Xylaria cf warburgii was identified as the causal agent of this disease in Taiwan and Lampung, Indonesia. However, the causal agent of sugarcane RBSR in South Sumatra, Indonesia, has still not been characterized. Here, we aimed to isolate and characterize the causal agent of sugarcane RBSR in South Sumatra based on morphological and molecular analyses. Accordingly, the fungal pathogen from diseased sugarcane in South Sumatra was isolated and the internal transcribed spacer region and morphological characteristics were used to identify the causal agent. Based on the morphological characteristics and phylogenetic tree generated using sequences of the ribosomal DNA internal transcribed spacers, Xylaria arbuscula was identified to be the causal agent of RBSR. Pathogenicity assay confirmed that symptoms caused by X. arbuscula infection resembled those observed in plant in the field. This is the first report of characterization of X. arbuscula causing sugarcane root and basal stem rot in Indonesia.
机译:根茎和基茎腐烂(RBSR)是一种新的严重疾病,影响印度尼西亚的甘蔗种植,可导致农作物产区,特别是南蓬和苏门答腊南部的农作物减产。这种病最早是在台湾描述的。根据形态特征,在台湾和印度尼西亚楠榜,已将Xylaria cf warburgii鉴定为该病的病原体。然而,在印度尼西亚南苏门答腊的甘蔗RBSR的病因仍未鉴定。在这里,我们旨在基于形态和分子分析,分离和表征南苏门答腊甘蔗RBSR的病原体。因此,从苏门答腊南部患病的甘蔗中分离出真菌病原体,并利用内部转录的间隔区和形态学特征鉴定病原。根据利用核糖体DNA内部转录间隔区序列生成的形态学特征和系统发育树,确定了Xylaria arbuscula是RBSR的病原体。致病性分析证实,由弓形虫感染引起的症状类似于在田间植物中观察到的症状。这是首次在印尼描述造成甘蔗根茎和基茎腐烂的X. arbuscula特征。

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