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Characterization of a 16SrI-B Subgroup Phytoplasma Associated with Sugarcane Leaf Yellows Disease in India

机译：印度甘蔗叶黄病相关的16SrI-B亚群植原体的表征

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摘要

Leaf mid-rib yellowing along with yellowing of adjoining lamina and development of irregular yellow patches on leaf lamina was observed on two sugarcane varieties (CoLk 94184, CoSe 92423) during 2013-2014 crop season. Phytoplasma association with leaf yellows and leaf lamina with yellow patches symptomatic samples was confirmed through PCR and nested PCR assays using phytoplasma specific primers P1/P6 followed by R16F2n/ R16R2. Sequencing and BLASTn analysis of ～ 1.2 kb product of 16Sr RNA gene fragments of the two phytopl-asmas shared 99 and 100 % sequence identity with strains of 'Candidates Phytoplasma asteris' (16SrI). Further characterization through in silico restriction enzyme digestion of 16Sr DNA using iPhy Classifier online tool assigned both the strains into subgroup 16SrI-B and phy-logenetic analyses confirmed that both the phytoplasmas were closely related to 16SrI group. This is the first report of association of 16SrI-B subgroup phytoplasma with sugarcane leaf yellows disease in India.
机译：在2013-2014作物季节期间，在两个甘蔗品种（CoLk 94184，CoSe 92423）上观察到叶片中肋变黄，以及邻接的叶片变黄以及叶片上不规则的黄色斑块的发展。通过使用植物质体特异性引物P1 / P6，然后是R16F2n / R16R2的PCR和巢式PCR分析，证实了与叶黄和叶板有黄色斑块症状样品的质体相关性。两个phytopl-asmas的16Sr RNA基因片段的〜1.2 kb产物的测序和BLASTn分析与“ Candidates Phytoplasma asteris”（16SrI）菌株具有99％和100％的序列同一性。通过使用iPhy分类器在线工具对16Sr DNA进行计算机限制性酶切消化，进一步鉴定了这两个菌株，并将它们都划分为16SrI-B亚组，并进行了植骨性分析，确认这两个植原体均与16SrI组密切相关。这是印度第一个将16SrI-B亚型植物质体与甘蔗叶黄病相关联的报道。

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  • 来源
    《Sugar Tech》 |2015年第2期|156-161|共6页
  • 作者

    Shailender Kumar; Ajay K. Tiwari; Somnath K. Holkar; S. K. Duttamajumder; G. P. Rao;

  • 作者单位

    Division of Plant Pathology, Indian Institute of Agricultural Research, New Delhi 110012, India;

    Sugarcane Research Institute, UP Council of Sugarcane Research, Shahjahanpur 242001, Uttar Pradesh, India;

    Division of Crop Protection, Indian Institute of Sugarcane Research, Lucknow 226002, Uttar Pradesh, India;

    Division of Crop Protection, Indian Institute of Sugarcane Research, Lucknow 226002, Uttar Pradesh, India;

    Division of Plant Pathology, Indian Institute of Agricultural Research, New Delhi 110012, India;

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  • 正文语种 eng
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  • 关键词

    Sugarcane leaf yellows phytoplasma; 'Candidates Phytoplasma asteris'; Phylogeny; 16SrI-B; YLD; YLS; SCLP;

    机译：甘蔗叶使植物质泛黄;'念珠菌植原体';系统发育16SrI-B;YLD;YLS;资深大律师;

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