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首页> 外文期刊>Theoretical and Applied Genetics >Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii
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Molecular markers linked to the apple scab resistance gene Vbj derived from Malus baccata jackii

机译：与苹果花斑病抗苹果黑星病基因Vbj连锁的分子标记

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摘要

Breeding for scab-resistant apple cultivars by pyramiding several resistance genes in the same genetic background is a promising way to control apple scab caused by the fungus Venturia inaequalis. To achieve this goal, DNA markers linked to the genes of interest are required in order to select seedlings with the desired resistance allele combinations. For several apple scab resistance genes, molecular markers are already available; but until now, none existed for the apple scab resistance gene Vbj originating from the crab apple Malus baccata jackii. Using bulk segregant analysis, three RAPD markers linked to Vbj were first identified. These markers were transformed into more reliable sequence-characterised amplified region (SCAR) markers that proved to be co-dominant. In addition, three SSR markers and one SCAR were identified by comparing homologous linkage groups of existing genetic maps. Discarding plants showing genotype–phenotype incongruence (GPI plants) plants, a linkage map was calculated. Vbj mapped between the markers CH05e03 (SSR) and T6-SCAR, at 0.6 cM from CH05e03 and at 3.9 cM from T6-SCAR. Without the removal of the GPI plants, Vbj was placed 15 cM away from the closest markers. Problems and pitfalls due to GPI plants and the consequences for mapping the resistance gene accurately are discussed. Finally, the usefulness of co-dominant markers for pedigree analysis is also demonstrated.
机译：通过在相同遗传背景中金字塔化多个抗性基因来培育抗sc病的苹果品种，是控制由真菌Venturia inaequalis引起的苹果sc病的有前途的方法。为了实现该目标，需要与目的基因连锁的DNA标记，以便选择具有所需抗性等位基因组合的幼苗。对于几种苹果黑星病抗性基因，分子标记已经可用。但是直到现在，关于蟹sc苹果苹果（Malus baccata jackii）的苹果抗ab疮病基因Vbj仍然不存在。使用批量分离器分析，首先鉴定了与Vbj连锁的三个RAPD标记。这些标记被转化为更可靠的序列特征性扩增区域（SCAR）标记，被证明是共同主导的。此外，通过比较现有遗传图谱的同源连锁群，鉴定出了三个SSR标记和一个SCAR。丢弃显示基因型-表型不一致的植物（GPI植物），计算连锁图。 Vbj映射在标记CH05e03（SSR）和T6-SCAR之间，距CH05e03为0.6 cM，距T6-SCAR为3.9 cM。在不去除GPI植物的情况下，将Vbj放置在距最接近的标记物15cM处。讨论了由GPI植物引起的问题和陷阱，以及准确定位抗性基因的后果。最后，还证明了共同标记对谱系分析的有用性。

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  • 来源
    《Theoretical and Applied Genetics》 |2004年第8期|1702-1709|共8页
  • 作者

    M Gygax; L Gianfranceschi; R Liebhard; M Kellerhals; C Gessler; A Patocchi;

  • 作者单位

    Plant Pathology Institute of Plant Sciences Swiss Federal Institute of Technology;

    Plant Pathology Institute of Plant Sciences Swiss Federal Institute of TechnologyDepartment of Biomolecular Sciences and Biotechnology University of Milano;

    Plant Pathology Institute of Plant Sciences Swiss Federal Institute of Technology;

    Agroscope FAW Wädenswil;

    Plant Pathology Institute of Plant Sciences Swiss Federal Institute of Technology;

    Plant Pathology Institute of Plant Sciences Swiss Federal Institute of Technology;

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