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首页> 外文期刊>Theoretical and Applied Genetics >Microsatellite markers for genome analysis in Brassica. II. Assignment of rapeseed microsatellites to the A and C genomes and genetic mapping in Brassica oleracea L.
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Microsatellite markers for genome analysis in Brassica. II. Assignment of rapeseed microsatellites to the A and C genomes and genetic mapping in Brassica oleracea L.

机译：用于芸苔属植物基因组分析的微卫星标记。二。油菜微卫星分配给甘蓝型油菜的A和C基因组以及遗传图谱。

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摘要

Microsatellites are highly polymorphic and efficient markers for the analysis of plant genomes. Primer specificity, however, may restrict the applicability of these markers even between closely related species for comparative mapping studies. We have demonstrated that the majority of microsatellites identified in oilseed rape (Brassica napus L; AC genome) correspond to loci which can be easily assigned to the A and C progenitor genomes. A study with 63 primer pairs has shown that 54% detect two loci, one from each genome, while 25% and 21%, respectively, are either A or C genome-specific. The distribution of rapeseed microsatellites in the C genome was investigated by genetic mapping in Brassica oleracea L. Ninety two dinucleotide microsatellites were screened for polymorphism in an F2 population derived from a cross between collard and cauliflower, for which an RFLP map has been constructed previously. Thirty three primer pairs (35.7%) have yielded either unspecific or no PCR products whereas the remaining primer pairs amplified one or more distinct loci. The level of polymorphism found in the mapping population was 49.2%. A total of 29 primer pairs disclosed 34 loci of which 31 are evenly distributed on 8 of the 9 B. oleracea linkage groups. For the remaining three markers linkage could not be established. Our results showed that microsatellite markers from the composite genome of B. napus can serve as a useful marker system in genetic studies and for plant-breeding objectives in B. oleracea.
机译：微卫星是用于植物基因组分析的高度多态性和高效标记。但是，引物特异性可能会限制这些标记的适用性，即使是在密切相关的物种之间进行比较作图研究也是如此。我们已经证明，在油菜（甘蓝型油菜L； AC基因组）中鉴定出的大多数微卫星对应于可以轻松分配给A和C祖细胞基因组的基因座。对63个引物对的研究表明，有54％的人检测到两个基因座，每个基因组一个，而分别有25％和21％的人对A或C基因组具有特异性。通过甘蓝型油菜的遗传作图研究了油菜籽微卫星在C基因组中的分布。筛选了九十二个二核苷酸微卫星在F2群体中的多态性，该群体来源于羽衣甘蓝和花椰菜之间的杂交，RFLP图具有以前建造的。 33对引物（占35.7％）产生了非特异性或无PCR产物，而其余的引物对则扩增了一个或多个不同的基因座。在作图群体中发现的多态性水平为49.2％。共有29个引物对公开了34个基因座，其中31个均匀分布在9个油菜芽孢杆菌连接基团中的8个上。对于其余三个标记，无法建立链接。我们的结果表明，来自甘蓝型油菜复合基因组的微卫星标记物可作为遗传研究中的有用标记物系统和油菜双歧杆菌的植物育种目标。

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  • 来源
    《Theoretical and Applied Genetics》 |2001年第5期|695-699|共5页
  • 作者

    B. Saal; J. Plieske; J. Hu; C. F. Quiros; D. Struss;

  • 作者单位

    Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) Corrensstraße 3 06466 Gatersleben Germany Present address: D. Struss Department of Pomology University of California Davis CA 95616 USA;

    Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) Corrensstraße 3 06466 Gatersleben Germany Present address: D. Struss Department of Pomology University of California Davis CA 95616 USA;

    Lipton Tomato Technology Center 2029 E. Harding Way Stockton CA 95205 USA;

    Department of Vegetable Crops University of California Davis CA 95616 USA;

    Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) Corrensstraße 3 06466 Gatersleben Germany Present address: D. Struss Department of Pomology University of California Davis CA 95616 USA;

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  • 关键词

    Key words Microsatellites; Brassica oleracea; Genetic mapping;

    机译：关键词微卫星;甘蓝;遗传定位;

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