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首页> 外文期刊>Theoretical and Applied Genetics >Molecular mapping of the rf1 gene for pollen fertility restoration in sorghum (Sorghum bicolor L.)
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Molecular mapping of the rf1 gene for pollen fertility restoration in sorghum (Sorghum bicolor L.)

机译：rf1基因在高粱花粉育性恢复中的分子作图

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摘要

We report the molecular mapping of a gene for pollen fertility in A1 (milo) type cytoplasm of sorghum using AFLP and SSR marker analysis. DNA from an F2 population comprised of 84 individuals was screened with AFLP genetic markers to detect polymorphic DNAs linked to fertility restoration. Fifteen AFLP markers were linked to fertility restoration from the initial screening with 49 unique AFLP primer combinations (+3/+3 selective bases). As many of these AFLP markers had been previously mapped to a high-density genetic map of sorghum, the target gene (rf1) could be mapped to linkage group H. Confirmation of the map location of rf1 was obtained by demonstrating that additional linkage group-H markers (SSR, STS, AFLP) were linked to fertility restoration. The closest marker, AFLP Xtxa2582, mapped within 2.4 cM of the target loci while two SSRs, Xtxp18 and Xtxp250, flanked the rf1 locus at 12 cM and 10.8 cM, respectively. The availability of molecular markers will facilitate the selection of pollen fertility restoration in sorghum inbred-line development and provide the foundation for map-based gene isolation.
机译：我们报告了使用AFLP和SSR标记分析的高粱A1（milo）型细胞质中花粉育性基因的分子定位。用AFLP遗传标记筛选来自84个个体的F2 群体的DNA，以检测与生育力恢复有关的多态性DNA。通过最初的筛选，使用49种独特的AFLP引物组合（+ 3 / + 3选择性碱基），将15个AFLP标记与恢复生育能力联系起来。由于许多此类AFLP标记先前已被定位到高粱的高密度遗传图谱上，因此目标基因（rf1）可以被定位于连锁组H。通过证明另外的连锁组- H标记（SSR，STS，AFL​​P）与生育力恢复相关。最接近的标记AFLP Xtxa2582定位在目标基因座的2.4 cM内，而两个SSR Xtxp18和Xtxp250分别位于rf1基因座的侧翼，分别位于12 cM和10.8 cM。分子标记的可用性将有助于选择高粱近交系中花粉繁殖力的恢复，并为基于图谱的基因分离提供基础。

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  • 来源
    《Theoretical and Applied Genetics》 |2001年第8期|1206-1212|共7页
  • 作者

    R. R. Klein; P. E. Klein; A. K. Chhabra; J. Dong; S. Pammi; K. L. Childs; J. E. Mullet; W. L. Rooney; K. F. Schertz;

  • 作者单位

    USDA-ARS Southern Plains Agricultural Research Center College Station TX 77845 USA e-mail: Bob@algodon.tamu.edu Fax: (979)-260-9333 Tel.: (979)-777-4470;

    Department of Biochemistry-Biophysics and Institute for Plant Genomics and Biotechnology Texas AM University College Station TX 77843 USA;

    Institute for Plant Genomics and Biotechnology Texas AM University College Station TX 77843 USA;

    Institute for Plant Genomics and Biotechnology Texas AM University College Station TX 77843 USA;

    Institute for Plant Genomics and Biotechnology Texas AM University College Station TX 77843 USA;

    Department of Biochemistry-Biophysics and Institute for Plant Genomics and Biotechnology Texas AM University College Station TX 77843 USA;

    Department of Biochemistry-Biophysics and Institute for Plant Genomics and Biotechnology Texas AM University College Station TX 77843 USA;

    Department of Soil and Crop Sciences Texas AM University College Station TX 77843 USA;

    USDA-ARS Southern Plains Agricultural Research Center College Station TX 77845 USA e-mail: Bob@algodon.tamu.edu Fax: (979)-260-9333 Tel.: (979)-777-4470;

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  • 正文语种 eng
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  • 关键词

    Keywords Sorghum (Sorghum bicolor L.); Fertility restoration; AFLP; Microsatellite;

    机译：高粱;生育力恢复;AFLP;微卫星;

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