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首页> 外文期刊>Theoretical and Applied Genetics >Phylogenetic and genomic relationships in Setaria italica and its close relatives based on the molecular diversity and chromosomal organization of 5S and 18S-5.8S-25S rDNA genes
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Phylogenetic and genomic relationships in Setaria italica and its close relatives based on the molecular diversity and chromosomal organization of 5S and 18S-5.8S-25S rDNA genes

机译：基于5S和18S-5.8S-25S rDNA基因的分子多样性和染色体组织的意大利Setaria及其近亲的系统发生和基因组关系

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摘要

We have analyzed the phylogenetic and genomic relationships in the genus Setaria Beauv. including diploid and tetraploid species, by means of the molecular diversity of the 5S rDNA spacer and chromosomal organization of the 5S and 18S-5.8S-25S rDNA genes. PCR amplification of the 5S rDNA sequences gave specific patterns. All the species studied here share a common band of about 340 bp. An additional band of an approximately 300-bp repeat unit was found for Setaria verticillata and the Chinese accessions of Setaria italica and Setaria viridis. An additional band of 450 bp was found in the sole species Setaria faberii. Fluorescent in situ hybridization was used for physical mapping of the 5S and 18S-5.8S-25S rDNA genes and showed that they are localized at two separate loci with no polymorphism of chromosome location among species. Two chromosome pairs carrying the 5S and 18S-5.8S-25S rDNA clusters can now be unambiguously identified using FISH. Phylogenetic trees based on the variation of the amplified 5S rDNA sequences showed a clear separation into four groups. The clustering was dependent on the genomic composition (genome A versus genome B) and confirmed the closest relationship of S. italica and S. viridis accessions from the same geographical region. Our results confirm previous hypotheses on the domestication centers of S. italica. They also show the wide difference between the A and B genomes, and even clarify the taxonomic position of S. verticillata.
机译：我们已经分析了Setaria Beauv属的系统发育和基因组关系。通过5S rDNA间隔区的分子多样性以及5S和18S-5.8S-25S rDNA基因的染色体组织，包括二倍体和四倍体物种。 5S rDNA序列的PCR扩增给出了特定的模式。此处研究的所有物种共有一个约340 bp的共同条带。发现了黄狗（Setaria verticillata）以及中国狗尾草（Setaria italica）和狗尾草（Setaria viridis）的另一个大约300 bp重复单元带。在唯一的Setaria faberii物种中发现了另一个450 bp的条带。荧光原位杂交技术用于5S和18S-5.8S-25S rDNA基因的物理定位，并显示它们位于两个独立的基因座，且物种之间的染色体位置没有多态性。现在可以使用FISH明确鉴定携带5S和18S-5.8S-25S rDNA簇的两个染色体对。基于扩增的5S rDNA序列的变异的系统发育树清楚地分为四个组。聚类取决于基因组组成（基因组A与基因组B），并证实了来自同一地理区域的意大利链球菌和绿色链球菌的亲缘关系最密切。我们的结果证实了关于意大利番茄的驯化中心的先前假设。它们还显示了A和B基因组之间的巨大差异，甚至阐明了黄褐葡萄球菌的分类位置。

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