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Hydrolysis of Cotton Cellulose by Engineered Cellulases from Trichoderma reesei

机译:里氏木霉工程纤维素酶水解棉纤维素

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摘要

We have characterized the activities of TC, EG-rich, and CBH-rich cellulases form T. reesei and have shown that their activities towards cotton fabrics are influenced by ionic strength and adsorbed ionic species as well as by temperature and pH. Adsorption and kinetic experiments confirm that increasing mechanical agitation favors EG attack by greatly increasing the availability of sites for EG adsorption. It is not clear whether this is a consequence of fiber fibrillation damage or of improved access to fiber surfaces deep within the fabric structure.
机译:我们已经表征了里氏木霉形成的富含TC,富含EG和富含CBH的纤维素酶的活性,并表明它们对棉织物的活性受离子强度和吸附的离子种类以及温度和pH值的影响。吸附和动力学实验证实,机械搅拌的增加通过大大增加EG吸附位点的可用性而有利于EG的攻击。尚不清楚这是纤维原纤化破坏的结果还是织物结构内部深处的纤维表面改善的进入的结果。

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