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首页> 外文期刊>Textile Research Journal >Hydrolysis of Cotton Cellulose by Engineered Cellulases from Trichoderma reesei
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Hydrolysis of Cotton Cellulose by Engineered Cellulases from Trichoderma reesei

机译：里氏木霉工程纤维素酶水解棉纤维素

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摘要

We have characterized the activities of TC, EG-rich, and CBH-rich cellulases from T. reesei and have shown that their activities towards cotton fabrics are influenced by ionic strength and adsorbed ionic species as well as by temperature and pH. Adsorption and kinetic experiments confirm that increasing mechanical agitation favors EG attack by greatly increasing the availability of sites for EG adsorption. It is not clear whether this is a consequence of fiber fibrillation damage or of improved access to fiber surfaces deep within the fabric structure. The enhanced rate of cellulolytic hydrolysis of mercerized cotton and the inhibitory effects on reactive and direct dyed fabrics are explained mainly in terms of increased or reduced availability of adsorption sites for CBHS and EGS. The implications for textile finishing are far-reaching. It is clear that a fabric's processing history (especially mercerizing and dyeing), construction, and level of applied mechanical agitation can be as important as the choice of enzyme composition and concentration in determining the consistency and quality of the end result.
机译：我们已经表征了来自里氏木霉的富含TC，富含EG和CBH的纤维素酶的活性，并表明它们对棉织物的活性受离子强度和吸附的离子种类以及温度和pH值的影响。吸附和动力学实验证实，机械搅拌的增加通过大大增加EG吸附位点的可用性而有利于EG的攻击。尚不清楚这是纤维原纤化破坏的结果还是织物结构内部深处的纤维表面改善的进入的结果。主要是通过增加或减少CBHS和EGS吸附位的可用性来解释丝光棉纤维素分解水解速率的提高以及对活性和直接染色织物的抑制作用。对纺织品整理的影响是深远的。显然，在确定最终结果的一致性和质量时，织物的加工历史（特别是丝光和染色），构造和施加的机械搅拌水平与选择酶的组成和浓度一样重要。

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