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首页> 外文期刊>The Analyst >Differentiation between normal and tumor vasculature of animal and human glioma by FTIR imaging
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Differentiation between normal and tumor vasculature of animal and human glioma by FTIR imaging

机译：FTIR成像区分动物和人神经胶质瘤的正常和肿瘤脉管系统

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Malignant gliomas are very aggressive tumors, highly angiogenic and invading heterogeneously thensurrounding brain parenchyma, making their resection very difficult. To overcome the limits of currentndiagnostic imaging techniques used for gliomas, we proposed using FTIR imaging, with a spatialnresolution from 6 to 10 mm, to provide molecular information for their histological examination, basednon discrimination between normal and tumor vasculature. Differentiation between normal and tumornblood vessel spectra by hierarchical cluster analysis was performed on tissue sections obtained fromnxenografted brain tumors of Rag-gamma mice 28 days after intracranial implantation of glioma cells,nas well as for human brain tumors obtained in clinics. Classical pathological examination andnimmunohistochemistry were performed in parallel to the FTIR spectral imaging of brain tissues. Firstnon the animal model, classification of FTIR spectra of blood vessels could be performed using spectralnintervals based on fatty acyl (3050–2800 cmu00021) and carbohydrate (1180–950 cmu00021) absorptions, with thenformation of two clusters corresponding to healthy and tumor parts of the tissue sections. Further datantreatments on these two spectral intervals provided interpretable information about the molecularncontents involved in the differentiation between normal and tumor blood vessels, the latter presentingna higher level of fatty acyl chain unsaturation and an unexpected loss of absorption from osidicnresidues. This classification method was further successfully tested on human glioma tissue sections.nThese findings demonstrate that FTIR imaging could highlight discriminant molecular markers tondistinguish between normal and tumor vasculature, and help to delimitate areas of correspondingntissue.
机译：恶性神经胶质瘤是非常具有侵略性的肿瘤，高度血管生成，然后异质性侵入周围的脑实质，因此很难切除。为了克服目前用于神经胶质瘤的影像学诊断技术的局限性，我们提出使用FTIR成像技术，其空间分辨率范围为6至10 mm，以提供其组织学检查的分子信息，而不是区分正常和肿瘤脉管系统。颅内植入神经胶质瘤细胞，鼻腔和临床上获得的人脑肿瘤后，对来自Rag-γ小鼠异种移植脑瘤的组织切片进行分级聚类分析，以区分正常血管和肿瘤血血管光谱。经典的病理检查和免疫组织化学与脑组织的FTIR光谱成像并行进行。首先，在动物模型中，可以使用基于脂肪酰基（3050–2800 cmu00021）和碳水化合物（1180–950 cmu00021）吸收的光谱间隔对血管的FTIR光谱进行分类，然后形成与健康和肿瘤部位相对应的两个簇组织切片。在这两个光谱区间上的进一步数据处理提供了有关正常和肿瘤血管分化中涉及的分子含量的可解释信息，后者呈现更高水平的脂肪酰基链不饱和度以及从osidicnresidues吸收的意外损失。这种分类方法在人脑胶质瘤组织切片上得到了进一步的成功测试。这些发现表明，FTIR成像可以突出区分正常和肿瘤脉管系统的区别性分子标记，并有助于划定相应组织的区域。

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    《The Analyst》 |2010年第12期|p.3052-3059|共8页
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    Katia Wehbe Raphael Pineau Sandrine Eimer Anne Vital Hugues Loiseau and Gérard Déléris;

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    aUniversitu0002e Victor Segalen Bordeaux 2, CNRS UMR 5084, 146 Rue Lu0002eoSaignat, 33076 Bordeaux-Cedex, France. E-mail: gerard.deleris@u-bordeaux2.frbINSERM U 920, Universitu0002e Bordeaux 1, Avenue des Facultu0002es, 33405Talence-Cedex, FrancecPathology Department, Bordeaux University Hospital, Bordeaux, FrancedNeurosurgery Department, Bordeaux University Hospital, Bordeaux,France† This article is part of a themed issue on Optical Diagnosis. This issueincludes work presented at SPEC 2010 Shedding Light on Disease:Optical Diagnosis for the New Millennium, which was held inManchester, UK June 26th – July 1st 2010.‡ Present address: Diamond Light Source Ltd., Harwell Science &Innovation Campus, Didcot, Oxfordshire OX11 0DE, UK.;

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