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首页> 外文期刊>The Analyst >SR-FTIR spectroscopy of renal epithelial carcinoma side population cells displaying stem cell-like characteristics
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SR-FTIR spectroscopy of renal epithelial carcinoma side population cells displaying stem cell-like characteristics

机译：肾上皮癌侧群细胞的SR-FTIR光谱显示干细胞样特征

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It is hypothesized that cells with stem cell-like properties may be influential in carcinogenesis,npossessing the ability to self-renew, produce differentiated daughter cells and resist environmental orntherapeutic injury. This has led to a surge in interest in identifying and characterizing the tumourninitiating or cancer stem cell (CSC) with the aim of discovering novel diagnostic and prognosticnmarkers and of understanding the basic biology with the ultimate aim of generating new therapeuticnapproaches and biomarkers. However, a major hurdle to this process has been the lack of a trulynspecific cancer stem cell biomarker allied to the rarity of these cells. This has led to problems inncharacterising these CSCs by traditional ‘-omic’ techniques. Using a renal carcinoma cell line model, wenshow that synchrotron radiation-Fourier transform infrared (SR-FTIR) spectroscopy is a suitable toolnto measure discrete differences in the biochemistry of small numbers of single-cells. Using thenchemometric techniques of Principal Component and Linear Discriminant Analysis (PCA and LDA)nfor multivariate reduction, biochemical differences between the cells from different sub-populationsnwere evaluated. Results found lipid and phosphodiester vibrations to be particularly goodndiscriminating markers in the spectra of these stem-like cells, relative to the more differentiated,nproliferating cells that make up the majority of the cell population.
机译：假设具有干细胞样特性的细胞可能在癌变中具有影响力，具有自我更新的能力，产生分化的子代细胞以及抵抗环境疗法的伤害。这导致人们对鉴定和表征肿瘤起源或癌症干细胞（CSC）的兴趣激增，目的是发现新颖的诊断和预后标记，并了解基本生物学，最终目的是产生新的治疗方法和生物标记。然而，该过程的主要障碍是缺乏与这些细胞的稀有性相关的真正特异性的癌症干细胞生物标记。这导致了通过传统的“组学”技术无法表征这些CSC的问题。 wenshow使用肾癌细胞系模型，表明同步加速器辐射-傅立叶变换红外（SR-FTIR）光谱是测量少量单细胞生物化学中离散差异的合适工具。使用主成分和线性判别分析（PCA和LDA）n的化学计量学技术进行多变量还原，评估了来自不同亚群的细胞之间的生化差异。结果发现，相对于构成大多数细胞群的分化程度更高的增殖细胞，脂质和磷酸二酯振动是这些干样细胞光谱中特别好的区分标记。

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    《The Analyst》 |2010年第12期|p.3133-3141|共9页
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    Caryn Hughes Matthew Liew Ashwin Sachdeva Paul Bassan Paul Dumas Claire A. Hart Mick D. Brown Noel W. Clarke and Peter Gardner;

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    aManchester Interdisciplinary Biocentre, University of Manchester, 131Princess Street, Manchester, UK M1 7DNbGenito-Urinary Cancer Research Group, School of Cancer and EnablingSciences, Paterson Institute for Cancer Research, University ofManchester, Christie Hospital NHS Trust, Manchester Academic HealthSciences Centre, Manchester, UK M20 4BXcSynchrotron Soleil, L’orme des Merisiers, Saint-Aubin, BP48 91192 Gifsur-Yvette Cedex, France† This article is part of a themed issue on Optical Diagnosis. This issueincludes work presented at SPEC 2010 Shedding Light on Disease:Optical Diagnosis for the New Millennium, which was held inManchester, UK June 26th–July 1st 2010.;

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