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首页> 外文期刊>The Analyst >Vibrational spectroscopy differentiates between multipotent and pluripotent stem cells
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Vibrational spectroscopy differentiates between multipotent and pluripotent stem cells

机译：振动光谱法可区分多能干细胞和多能干细胞

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摘要

Over the last few years, there has been an increased interest in the study of stem cells in biomedicine forntherapeutic use and as a source for healing diseased or injured organs/tissues. More recently,nvibrational spectroscopy has been applied to study stem cell differentiation. In this study, we have usednboth synchrotron based FTIR and Raman microspectroscopies to assess possible differences betweennhuman pluripotent (embryonic) and multipotent (adult mesenchymal) stem cells, and how O2nconcentration in cell culture could affect the spectral signatures of these cells. Our work shows thatninfrared spectroscopy of embryonic (pluripotent) and adult mesenchymal (multipotent) stem cells havendifferent spectral signatures based on the amount of lipids in their cytoplasm (confirmed withncytological staining). Furthermore, O2 concentration in cell culture causes changes in both the FTIRnand Raman spectra of embryonic stem cells. These results show that embryonic stem cells might benmore sensitive to O2 concentration when compared to mesenchymal stem cells. While vibrationalnspectroscopy could therefore be of potential use in identifying different populations of stem cells furthernwork is required to better understand these differences.
机译：在过去的几年中，对干细胞在生物医学用于治疗方面以及作为治愈患病或受伤的器官/组织的来源的研究越来越引起人们的兴趣。最近，振动光谱已被用于研究干细胞的分化。在这项研究中，我们已经使用基于同步加速器的FTIR和拉曼光谱技术来评估人类多能干（胚胎）干细胞和多能干（成人间充质）干细胞之间的可能差异，以及细胞培养物中O2n浓度如何影响这些细胞的光谱特征。我们的工作表明，胚胎干细胞（多能干细胞）和成年间质干细胞（多能干细胞）的红外光谱根据其细胞质中脂质的含量而具有不同的光谱特征（经放射学证实）。此外，细胞培养物中的氧气浓度会导致胚胎干细胞的FTIRn和拉曼光谱发生变化。这些结果表明，与间充质干细胞相比，胚胎干细胞可能对O2浓度更敏感。因此，振动波谱法可能在识别干细胞的不同群体方面具有潜在的用途，需要进一步努力以更好地理解这些差异。

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    《The Analyst》 |2010年第12期|p.3126-3132|共7页
  • 作者

    Jacek Klaudiusz Pijanka Deepak Kumar Tina Dale Ibraheem Yousef Gary Parkes Valérie Untereiner Ying Yang Paul Dumas David Collins Michel Manfait Ganesh Dhruvananda Sockalingum Nicholas Robert Forsyth and Josep Sulé-Suso;

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    aInstitute for Science and Technology in Medicine, Guy Hilton ResearchCentre, Keele University, Stoke on Trent, UKbSOLEIL Synchrotron, BP48, L’Orme des Merisiers, Gif sur Yvette,FrancecDepartment of Histopathology, Central Pathology Laboratory, UniversityHospital of North Staffordshire, Stoke on Trent, UKdMu0002eDIAN, CNRS UMR6237-MEDyC, Universitu0002e de Reims Champagne-Ardenne, UFR de Pharmacie, 51096 Reims cedex, FranceeSchool of Computing and Mathematics, Keele University, Keele, UKfCancer Centre, University Hospital of North Staffordshire, Stoke onTrent, Staffordshire, ST4 6QG, UK† This article is part of a themed issue on Optical Diagnosis. This issueincludes work presented at SPEC 2010 Shedding Light on Disease:Optical Diagnosis for the New Millennium, which was held inManchester, UK June 26th–July 1st 2010.;

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