• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Kasetsart Journal >Effects of Acute and Chronic Gamma Irradiation on Tissue Culture of Cryptocoryne wendtii 'brown'
【24h】

Effects of Acute and Chronic Gamma Irradiation on Tissue Culture of Cryptocoryne wendtii 'brown'

机译：急性和慢性伽马射线辐照对温氏隐球菌“棕色”组织培养的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The value of aquatic plant exports from Thailand is rising and there is a demand for interesting new varieties of aquatic plants. The objective of this research was to find the appropriate dose of gamma irradiation to induce mutations in Cryptocoryne wendtii "brown." C. wendtii "brown" was cultured aseptically on MS medium supplemented with BA (2mg/l) and NAA (0.25 mg/l). Samples of the tissue-cultured plantlets were exposed to acute gamma radiation using a Mark I Gamma Irradiator with a Cesium-137 source at doses of 0, 10, 20, 30, 40 and 50 Gy. Other samples were exposed to chronic gamma radiation in a gamma room using a Cobalt-60 source at the Gamma Irradiation Service and Nuclear Technology Research Center of the Faculty of Science, Kasetsart University. The samples were placed at 1.5 and 2.5 m from the source with a dose rate of 1.614 and 0.537 Gy/hr, respectively. The samples at 1.5 m received a total dose of 238.87 and 477.74 Gy (0 for the control) and those at 2.5 m received a total dose of 10.75, 21.50, 32.24 and 42.99 Gy (0 for the control). The number of dead and surviving plantlets and the number of new shoots were recorded at 45 days after irradiation and compared with the controls to calculate the LD_(50(45)) and GR_(50(45)). Under acute irradiation, the LD(50(45)) and GR_(50(45)) for C. wendtii "brown" were found to be 23 and 20 Gy, respectively. Some variations were observed, such as clumping, dwarfism, darker brown leaves, furled leaves and narrow leaves. Under chronic irradiation it was not possible to calculate the LD_(50(45)) or GR_(50(45)) because at 1.5 m from the source all the samples died and at 2.5 m from the source more than 50% of the samples survived. Some variations were observed, such as shorter plants, green leaves, increased branching, shorter petioles and longer, narrower leaves of a greenish-brown color.
机译：从泰国出口的水生植物的价值正在上升，对有趣的水生植物新品种的需求也在增加。这项研究的目的是找到合适剂量的伽马射线辐照以诱导温氏隐球菌“棕色”中的突变。在添加了BA（2mg / l）和NAA（0.25mg / l）的MS培养基上无菌培养温氏梭菌。使用带有铯137源的Mark Iγ辐照器以0、10、20、30、40和50 Gy的剂量将组织培养的小苗样品暴露于急性γ辐照。其他样品在Kasetsart大学理学院的伽玛辐照服务和核技术研究中心使用Cobalt-60光源在伽玛室内暴露于慢性伽玛辐射。将样品放置在距光源1.5 m和2.5 m处，剂量率分别为1.614和0.537 Gy / hr。 1.5 m的样品的总剂量为238.87和477.74 Gy（对照为0），而2.5 m的样品的总剂量为10.75、21.50、32.24和42.99 Gy（对照为0）。在照射后45天记录死亡和存活的幼苗的数量以及新芽的数量，并与对照进行比较以计算LD_（50（45））和GR_（50（45））。在急性照射下，发现温氏梭菌“棕色”的LD（50（45））和GR_（50（45））分别为23 Gy和20 Gy。观察到一些变化，例如结块，侏儒症，较深的褐色叶子，卷曲的叶子和狭窄的叶子。在长期照射下，不可能计算LD_（50（45））或GR_（50（45）），因为在距源1.5 m处所有样本均死亡，距源2.5 m处超过50％的样本死亡幸存下来。观察到一些变化，例如较短的植物，绿叶，增加的分支，较短的叶柄和较长，较窄的绿褐色叶子。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号