• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Kasetsart Journal >Effect of Sucrose and ABA on Survival Rate of Cryopreserved Protocorms of Dendrobium gratiosissimum Rchb.f.
【24h】

Effect of Sucrose and ABA on Survival Rate of Cryopreserved Protocorms of Dendrobium gratiosissimum Rchb.f.

机译：蔗糖和ABA对低温保存的石end石ch原皮低温保存率的影响。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Dendrobium gratiosissimum Rchb.f. is a rare wild orchid. Cryopreservation is an alternative and important tool for ex situ preservation of orchid germplasm. In this study, protocorms of D. gratiosissimum were induced and cultured on solidified New Dogashima (ND) medium containing 0.3 mg/1 NAA for two months. These protocorms were used as explants in cryopreservation using an encapsulation -dehydration method. For treatment with ABA, encapsulated protocorms were precultured in liquid ND medium containing various concentrations of ABA made up of 0,0.01, 1 and 10 mg/1. For the sucrose treatment, beads were precultured in a semi-solid ND medium containing 0.25 M sucrose in the dark for one week at 4℃ and then transferred into liquid ND medium containing 0, 0.5,0.75 and 1 M of sucrose and then cultured for two days before dehydration. The highest percentage regrowth of cryopreserved explants was observed when precultured in the liquid ND medium supplemented with 1 mg/1 ABA for one week and then dehydrated with silica gel in a laminar air-flow for five hours prior to immersion in liquid nitrogen for one hour. Regrowth ability testing performed after two weeks of treatment produced a rate of regrowth of 67% ± 0.5. Consequently, a simple and reliable protocol for the cryopreservation of D. gratiosissimum was clearly revealed in this study.
机译：石end铁皮石ch Rchb.f.是一种罕见的野生兰花。低温保存是兰花种质异地保存的另一种重要手段。在这项研究中，诱导了D. gratiosissimum的原球茎，并在含有0.3 mg / 1 NAA的凝固的新多吉岛（ND）培养基上培养了两个月。这些原球茎在使用包囊-脱水方法冷冻保存时用作外植体。为了用ABA处理，将封装的原球茎在液体ND培养基中预培养，该培养基含有各种浓度的ABA，浓度分别为0、0.01、1和10 mg / 1。对于蔗糖处理，将珠子在含有0.25 M蔗糖的半固态ND培养基中于4℃黑暗中预培养1周，然后转移至含有0、0.5、0.75和1 M蔗糖的液态ND培养基中，然后进行培养。脱水前两天。当在补充有1 mg / 1 ABA的液体ND培养基中预培养1周，然后在层流中用硅胶脱水5小时，然后浸入液氮中1小时后，观察到冷冻保存的外植体再繁殖的百分比最高。 。治疗两周后进行的再生能力测试产生了67％±0.5的再生率。因此，这项研究清楚地揭示了一种简单而可靠的方法，用于冷冻保存D. gratiosissimum。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号