...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Acta Horticulturae >Effect of sucrose on in vitro germplasm preservation in Dendrobium friedericksianum Rchb.f.
【24h】

Effect of sucrose on in vitro germplasm preservation in Dendrobium friedericksianum Rchb.f.

机译：绥科斯在Friedericksianf RCHB.FB中的体外先牙保存的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The present study was conducted to examine the effect of sucrose on the reduction of in vitro preservation of Dendrobium friedericksianum Rchb.f. seedlings after prolonged subculture in Vacin and Went (1949) (VW) medium supplemented with different concentrations of sucrose. D. friedericksianum is a native Thai wild orchid found in the eastern provinces of Thailand, especially in Chanthaburi Province. Currently, it is a highly endangered species because of illegal forest collection, decreasing rate ofnatural breeding, and environmental changes leading to a higher risk of extinction. The aim of the present study was to develop an effective protocol for in vitro storage of this species. Plantlets of D. friedericksianum were cultured on VW media supplemented with 0, 2, 4, 6, 8, and 10% sucrose. Plant height decreased when 8 and 10% sucrose was applied (decrease of 2.25 and 1.97 cm, respectively), which was significantly higher than the height decrease for those provided with 0, 2, 4, and 6% sucrose (decrease of 2.85, 4.75, 3.27, and 2.75 cm, respectively). Plants treated with 10% sucrose could be preserved for 6 months without subculture and plant height was decreased by only 2.78 cm. The pseudo-bulb diameter when treated with 10% sucrose was 0.08 cmhigher than that treated with the basal medium (2% sucrose).
机译：进行了本研究以检测蔗糖对石斛兰脆病床的体外保存减少的影响。幼苗在慢性培养后延长脱殖，并进行（1949）（VW）培养基，补充有不同浓度的蔗糖。 D. Friedericksianum是在泰国东部省份发现的原生泰国野生兰花，特别是在朱anthaburi省。目前，由于非法森林收集，育种率降低，环境变化导致濒临灭绝的风险更高，这是一种高度濒临灭绝的物种。本研究的目的是为该物种的体外储存制定有效的方案。 D.Friedericksianum的Plantlets在补充有0,2,4,6,8和10％蔗糖的VW培养基上培养。当施加8和10％蔗糖时（分别减少2.25和1.97cm），植物高度减少，这显着高于提供0,2,4和6％蔗糖（减少2.85,4.75 ，3.27和2.75厘米）。用10％蔗糖处理的植物可以保存6个月而不会转移，并且植物高度仅降低2.78厘米。用10％蔗糖处理时的伪灯泡直径比用基础培养基（2％蔗糖）处理0.08 cmHigher。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号