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Identifying Qtls For Fiber Content And Agronomic Characters In Sugarcane Using Aflp Markers

机译:使用Aflp标记鉴定甘蔗中纤维含量和农艺性状的Qtls

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摘要

Sugarcane is a highly complex poly-aneuploid, heterozygous, interspecific polyploid and cultivars commonly have over 100 chromosomes. The population of 168 progenies from a cross between a sugarcane commercial variety (K 93-207) and a S. spontaneum clone (MPT 97-1) were evaluated in a replicated field trial. Stalk diameter and tillering were recorded at harvesting and subsequently plant material was evaluated for fiber content. Clones were significantly different (P<0.01) for all traits analyzed. A total of 180 AFLP simplex markers were used to analyze the inheritance of quantitative trait loci (QTLs) for stalk diameter, tillering and fiber content. Thirty putative QTLs from the simplex markers were identified for these three traits. Each QTL explained from 3.7 to 10.7% of the variation for stalk diameter and 3.7 to 9.9% for fiber content. For tillering each QTL explained from 4-8% of the variation. K 93-207 contributed a positive effect to the QTLs for stalk diameter and a negative effect for the QTLs for fiber content and tillering. MPT 97-1 contributed a negative effect to the QTLs for stalk diameter and a positive effect for fiber content and tillering. Ten of the QTLs from both parents were identified as contributing to more than one trait. However, these markers were not available for all parts of this very highly complex genome. Further work will be needed to identify more markers in this population.
机译:甘蔗是高度复杂的多异倍体,杂合子,种间多倍体,品种通常具有100多个染色体。在重复的田间试验中评估了来自甘蔗商品品种(K 93-207)和自发链球菌克隆(MPT 97-1)之间杂交的168个子代。在收获时记录茎的直径和分ing,随后评估植物材料的纤维含量。对于所有分析的性状,克隆均具有显着差异(P <0.01)。总共180个AFLP单工标记用于分析茎秆直径,分till和纤维含量的数量性状基因座(QTL)的遗传。从这三个性状中鉴定出来自单纯形标记的三十个假定的QTL。每个QTL的茎直径变化为3.7%至10.7%,纤维含量为3.7%至9.9%。对于分till,每个QTL解释了变异的4-8%。 K 93-207对茎秆直径的QTL有积极影响,而对纤维含量和分till的QTL有负面影响。 MPT 97-1对茎秆直径的QTL有负面影响,对纤维含量和分till有正面影响。父母双方的QTL中有10个被认为有助于一个以上的特征。但是,这些标记物不适用于此非常复杂的基因组的所有部分。需要进一步的工作来确定该人群中的更多标记。

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