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首页> 外文期刊>The Kasetsart Journal >Molecular Taxonomy Of Acetobacter Syzygii Sku19 And Characterization Of Its Acetic Acid Adapted Strains
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Molecular Taxonomy Of Acetobacter Syzygii Sku19 And Characterization Of Its Acetic Acid Adapted Strains

机译：醋杆菌Sku19的分子分类学及其醋酸适应菌株的表征

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摘要

Acetobacter species are one of the most suitable bacteria for the proteomic analysis of proteins involved in stress response or adaptation. Among 129 isolates of thermotolerant acetic-acid bacteria, two isolates SKU19 and SKU123 were selected as an acetic-acid sensitive and acetic-acid resistant strain, respectively. Molecular taxonomy of these isolates indicated that SKU19 and SKU123 were Acetobacter syzygii and A. pasteurianus, respectively. To elucidate an adaptive response to acetic acid, acetic-acid-adapted strains were isolated from sequential cultivations of the acetic-acid sensitive strain, A. syzygii SKU19, in a medium containing 1 % acetic acid. The adapted variants could be divided into two groups based on their growth and ability to further oxidize acetate. The first group consisted of cells with increased overoxidation (rapid acetate oxidizers), while the second group contained cells with increased stability to acetate (slow acetate oxidizers). The membrane-bound quinoprotein alcohol dehydrogenase (PQQ-ADH) and aldehyde dehydrogenase (ALDH) activities of these adapted strains were higher than those of the wild type. The result corresponded well with the increased amount of protein with a molecular mass of 72-80 (subunit I) and 44-54 kDa (subunit II) when the organism was cultivated in a medium containing acetic acid. This study confirmed that acetic-acid resistance in acetic-acid bacteria is conferred by several mechanisms, such as acetate assimilation and PQQ-ADH functions.
机译：醋杆菌属是最适合用于蛋白质组学分析涉及应激反应或适应的蛋白质的细菌之一。在耐热乙酸细菌的129个分离株中，分别选择了两个分离株SKU19和SKU123作为对乙酸敏感和对乙酸有抵抗力的菌株。这些分离物的分子分类学表明，SKU19和SKU123分别是合酶醋杆菌和巴氏杆菌。为了阐明对乙酸的适应性反应，从乙酸敏感菌株A.syzygii SKU19的连续培养物中分离乙酸适应菌株，该菌株在含1％乙酸的培养基中。根据它们的生长和进一步氧化乙酸盐的能力，适应的变体可以分为两组。第一组由过度氧化增加的细胞（快速乙酸氧化剂）组成，而第二组包含对乙酸稳定的细胞（缓慢的乙酸氧化剂）。这些适应菌株的膜结合喹蛋白醇脱氢酶（PQQ-ADH）和醛脱氢酶（ALDH）活性均高于野生型。当该生物体在含乙酸的培养基中培养时，结果与分子量为72-80（亚基I）和44-54 kDa（亚基II）的蛋白质的增加量很好地吻合。这项研究证实，乙酸细菌中的乙酸抗性是由多种机制赋予的，例如乙酸同化和PQQ-ADH功能。

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