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首页> 外文期刊>The Kasetsart Journal >Isolation, Screening and Identification of Mannanase Producing Microorganisms
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Isolation, Screening and Identification of Mannanase Producing Microorganisms

机译：产生甘露聚糖酶的微生物的分离，筛选和鉴定

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摘要

Mannan is used in many industries and known to be the major polysaccharides of legume seeds, coconut kernel and tubers of konjac. Several bacteria and fungi have been reported to produce mannanase. In this paper, 19 bacteria and 4 fungi showing mannanase production were isolated from twenty-three soil samples. Isolate NT 6.7 showed high mannanase activity (0.306 unit/ml), displayed broad inhibition to pathogens; Salmonella serovar Enteritidis S003 and Escherichia coli E010, and could promote growth of probiotic Lactobacillus reuteri AC5. The strain was identified by morphological, physiological, biochemical (API 50 CHB, 99.5%identity) and 16 S rDNA sequence (99%identity) tests as Bacillus circulans. The mannanase activity from this strain had optimum pH of 7.0-9.0, optimum temperature at 50℃ and high stability at 50℃. The results suggested mannanase produced from B. circulans NT 6.7 had interesting properties and could be applied in prebiotic preparation.
机译：甘露聚糖用于许多行业，并且已知是豆类种子，椰子仁和魔芋块茎的主要多糖。据报道有几种细菌和真菌可产生甘露聚糖酶。本文从23个土壤样品中分离出19种细菌和4种显示甘露聚糖酶产生的真菌。分离株NT 6.7显示出较高的甘露聚糖酶活性（0.306单位/ ml），对病原体具有广泛的抑制作用。沙门氏菌肠炎沙门氏菌S003和大肠杆菌E010，并可以促进益生菌路透乳杆菌AC5的生长。通过形态，生理，生化（API 50 CHB，99.5％同一性）和16 S rDNA序列（99％同一性）测试将该菌株鉴定为圆形芽孢杆菌。该菌株的甘露聚糖酶活性最适pH为7.0-9.0，最适温度为50℃，最高稳定性为50℃。结果表明，由圆环芽孢杆菌NT 6.7产生的甘露聚糖酶具有有趣的性质，可用于益生元制备中。

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