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首页> 外文期刊>The Kasetsart Journal >Histology and Glycoconjugates Histochemistry in the Small and Large Intestinal Epithelium of the Malayan Pangolin, Manis javanica
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Histology and Glycoconjugates Histochemistry in the Small and Large Intestinal Epithelium of the Malayan Pangolin, Manis javanica

机译：马来亚穿山甲，马来亚爪哇小肠和大肠上皮的组织学和糖缀合物组织化学

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摘要

Histology and glycoconjugates histochemistry in the small and large intestinal epithelium of the Malayan Pangolin (Manis javanica) were studied by means of histological and histochemical techniques. The staining procedures employed were Hematoxylin-Eosin (H & E), Alcian Blue (AB) pH 2.5, Periodic Acid-Schiff (PAS), AB pH 2.5-PAS and lectin including penut agglutinin (PNA), Ulex europeus agglutinin-Ⅰ (UEA-Ⅰ), wheat germ agglutinin (WGA) and Maackia amurensis (MAL-Ⅱ). The intestinal canal displayed no divivsion between small and large intestine, other than a continuous enlargement, and there was no cecum. The mucous membrane of the pangolin small intestine was thrown into high villi and long crypt of Lieberkuhn. While, the mocosa of large intestine lied in a series of irregular fold, not villi. The mucosa of both intestine was covered by simple columnar epithelium in which 2 type of cells were encountered columnar and goblet cells. The mucous granule of goblet cells in small intestine were found to contain primarily neutral glycoconjugates with galactose and sialic acid residues. On the contrary, the mucous epithelium of large bowel involved predominantly acid sulfated glycoconjugates with N-acetylglucosamine, fucose and sialic acid residues. Furthermore, the nature of glycoconjugates in mucous epithelium of both intestine was found to change during the upward migration from deep to superficial part.
机译：通过组织学和组织化学技术研究了马来亚穿山甲（Manis javanica）小肠和大肠上皮的组织学和糖缀合物的组织化学。所用的染色方法为苏木精-曙红（H＆E），pH 2.5的阿尔辛蓝（AB），高碘酸席夫（PAS），AB pH 2.5-PAS和包括凝集素在内的凝集素（PNA），欧洲紫胶凝集素Ⅰ（ UEA-Ⅰ），小麦胚芽凝集素（WGA）和Maackia amurensis（MAL-Ⅱ）。除连续扩大外，小肠和大肠之间均无分裂，也没有盲肠。穿山甲小肠的粘膜被扔进Lieberkuhn的高绒毛和长隐窝中。同时，大肠的粘膜位于一系列不规则的褶皱中，而不是绒毛。两个肠的粘膜被简单的柱状上皮覆盖，其中遇到了两种类型的细胞：柱状和杯状细胞。发现小肠中杯状细胞的粘液颗粒主要含有带有半乳糖和唾液酸残基的中性糖缀合物。相反，大肠的粘膜上皮主要是带有N-乙酰氨基葡萄糖，岩藻糖和唾液酸残基的酸性硫酸化糖缀合物。此外，发现在两个肠的粘膜上皮中糖缀合物的性质在从深部向表层的向上迁移期间改变。

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