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首页> 外文期刊>Chromosome research: An international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology >Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting.
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Chromosomal rearrangements underlying karyotype differences between Chinese pangolin (Manis pentadactyla) and Malayan pangolin (Manis javanica) revealed by chromosome painting.

机译：染色体绘画揭示了中国穿山甲（Manis pentadactyla）和马来亚穿山甲（Manis javanica）之间的核型差异的染色体重排。

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摘要

The Chinese pangolin (Manis pentadactyla), a representative species of the order Pholidota, has been enlisted in the mammalian whole-genome sequencing project mainly because of its phylogenetic importance. Previous studies showed that the diploid number of M. pentadactyla could vary from 2n = 36 to 42. To further characterize the genome organization of M. pentadactyla and to elucidate chromosomal mechanism underlying the karyotype diversity of Pholidota, we flow-sorted the chromosomes of 2n = 40 M. pentadactyla, and generated a set of chromosome-specific probes by DOP-PCR amplification of flow-sorted chromosomes. A comparative chromosome map between M. pentadactyla and the Malayan pangolin (Manis javanica, 2n = 38), as well as between human and M. pentadactyla, was established by chromosome painting for the first time. Our results demonstrate that seven Robertsonian rearrangements, together with considerable variations in the quantity of heterochromatin and in the number of nucleolar organizer regions (NORs) differentiate the karyotypes of 2n = 38 M. javanica and 2n = 40 M. pentadactyla. Moreover, we confirm that the M. javanica Y chromosome bears one NOR. Comparison of human homologous segment associations found in the genomes of M. javanica and M. pentadactyla revealed seven shared associations (HSA 1q/11, 2p/5, 2q/10q, 4p+q/20, 5/13, 6/19p and 8q/10p) that could constitute the potential Pholidota-specific signature rearrangements.
机译：中国穿山甲（Manis pentadactyla）是Pholidota的代表种，由于其在系统进化上的重要性，已被纳入哺乳动物全基因组测序项目。先前的研究表明，五芒星分枝杆菌的二倍体数可以在2n = 36到42之间变化。为了进一步表征五角星分枝杆菌的基因组组织并阐明Pholidota核型多样性的染色体机制，我们对2n染色体进行了流分选= 40 M. pentadactyla，并通过DOP-PCR扩增分流后的染色体生成了一组染色体特异性探针。首次通过染色体画法建立了五芒藻和马来亚穿山甲之间的比较染色体图（Manis javanica，2n = 38），以及人与五芒藻之间的比较。我们的结果表明，七个罗伯逊重排，以及异染色质的数量和核仁组织区（NORs）数量的显着差异，区分了2n = 38 M. javanica和2n = 40 M pentadactyla的核型。此外，我们确认爪哇M. Y染色体带有一个NOR。比较人类爪哇分枝杆菌和五角分枝杆菌基因组中的人类同源节段关联，揭示了七个共有的关联（HSA 1q / 11、2p / 5、2q / 10q，4p + q / 20、5 / 13、6 / 19p和8q / 10p），可能构成潜在的幽灵特定标记重排。

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