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首页> 外文期刊>The Kasetsart Journal >Cloning and Molecular Characterization of α- and β- Amylase Genes from Cassava (Manihot esculenta Crantz)
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Cloning and Molecular Characterization of α- and β- Amylase Genes from Cassava (Manihot esculenta Crantz)

机译：木薯α-淀粉酶和β-淀粉酶基因的克隆及分子鉴定

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摘要

Starch is the primary storage polysaccharide in plants and can be degraded by amylolytic enzymes. Starch-degrading enzymes can be classified into α-amylase (1, 4-α-D-glucan glucanohydrolase; EC 3.2.1.1) and β-amylase (1, 4-α-D-glucan glucanohydrolase; EC 3.2.1.2). Two clones encoding α-amylase and β-amylase from leaves of Thai cassava were isolated and sequenced. The partial clones were 705 and 1,960 nucleotides in length, respectively. Total RNA was extracted from several parts of the cassava plants and analyzed for the presence and level of expression of the two genes by using PCR DIG-labeled α-amylase and β-amylase probes. The highest expression of the α-amylase gene was observed in storage roots, lower but detectable expression was in leaves and stems. On the other hand, the β-amylase gene was observed for highly expressed in leaves and storage roots. The copy number of these genes was determined using Southern analysis indicated that α-amylase is a single copy gene, while β-amylase is present in multiple copies in the cassava genome.
机译：淀粉是植物中的主要贮藏多糖，可以被淀粉分解酶降解。淀粉降解酶可分为α-淀粉酶（1，4-α-D-葡聚糖葡聚糖水解酶； EC 3.2.1.1）和β-淀粉酶（1，4-α-D-葡聚糖葡聚糖水解酶； EC 3.2.1.2）。分离并测序了来自泰国木薯叶片的两个编码α-淀粉酶和β-淀粉酶的克隆。部分克隆的长度分别为705和1960个核苷酸。从木薯植物的几个部位提取总RNA，并使用PCR DIG标记的α-淀粉酶和β-淀粉酶探针分析两个基因的存在和表达水平。在贮藏根部观察到α-淀粉酶基因的最高表达，在叶和茎中则较低，但可检测到。另一方面，观察到β-淀粉酶基因在叶和贮藏根中高表达。使用Southern分析确定这些基因的拷贝数，表明α-淀粉酶是单拷贝基因，而β-淀粉酶以木薯基因组中的多个拷贝存在。

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