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首页> 外文期刊>The Kasetsart Journal >Isolation and Identification of Mycorrhizal Fungi from Eleven Terrestrial Orchids
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Isolation and Identification of Mycorrhizal Fungi from Eleven Terrestrial Orchids

机译：十一种陆生兰花的菌根真菌的分离与鉴定

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摘要

Mycorrhizal fungi were isolated from eleven terrestrial orchid species, namely Calanthe rubens, Calanthe rosea, Cymbidium sinense, Cymbidium tracyanum, Goodyera procera, Ludisia discolor, Paphiopedilum concolor, P. exul, P. godefroyae, P. niveum and P. villosum. Healthy roots of orchid hosts were collected from various parts of the country. Isolation of mycorrhizal fungi from pelotons was carried out using a modification of Masuhara and Katsuya method. Identification was based on morphological characteristics. Nuclei were stained with safranin O using Bandoni's method. Seven genera and fourteen species of mycorrhizal fungi were identified: Ceratorhizacerealis, C. goodyerae-repentis, C.pernacatena, C. ramicola, Ceratorhiza sp., Epulorhiza calendulina, E. repens, Rhizoctonia globularis, Sistotrema sp., Trichosporiella multisporum, Tulasnella sp. Waitea circinata and two Rhizoctonia species. Nuclear staining revealed that all strains were binucleate except for W. circinata which was multinucleate. Pure cultures were maintained on PDA slant and liquid paraffin at the Culture Collection, Department of Plant Pathology, Kasetsart University.
机译：从11种陆生兰花物种中分离了菌根真菌，这些物种分别为Calanthe rubens，Calanthe rosea，Cymbidium sinense，Cymbidium tracyanum，Goodyera procera，Ludisia discolor，兜兰兜兰，P。exul，P。godefroyae，P。niveum和P.villosum。兰花寄主的健康根系来自该国各地。使用改良的Masuhara和Katsuya方法从果胶中分离菌根真菌。鉴定基于形态特征。使用班多尼法将番红花O染色核。鉴定了7个属和14种菌根真菌：蕨类植物，好叶念珠菌，C.pernacatena，毛果念珠菌，Ceratorhiza sp。，Epulorhiza calendulina，E。repens，球状Rhizoctonia globularis，Sistotrema sp。，Trichosporiella sp.sporum 。 Waitea circinata和两个Rhizoctonia种。核染色表明，除W.circinata多核外，所有菌株均为双核。在Kasetsart大学植物病理学系的Culture Collection中，将纯培养物保持在PDA倾斜液和液体石蜡上。

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