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Biotreatment of High Fat and Oil Wastewater by Lipase Producing Microorganisms

机译:脂肪酶生产微生物对高油脂废水的生物处理

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Studies on biotreatments of high fat and oil wastewater by selected lipase producing bacteria were carried out in two experiments with two groups of bacteria, single culture and mixed culture. In the first experiment, wastewater of bakery industry was treated with 4 bacteria isolates. Results showed that grease in the form of fat and oil and COD decreased remarkably with treatments. With single culture, the removal of fat and oil and COD were 73%-88% and 81 %-99% during 7 days treatment. Isolates KUL8 and KUL39 showed better activities. However, better treatments were found in experiment treated with single culture than using mixed culture. In the second experiment, wastewater of palm oil and bakery industries were treated with 6 isolates within 48 hours. All 6 isolates showed better degradation in palm oil wastes than bakery wastes. However, KUL8 and KUL39 still showed better degradation activities. Both isolates can removed fat and oil by 87.7% and 80.6% in palm oil wastewater and 70% and 64% in bakery wastes respectively. The decreasing of COD was found to be 90%-96%. When mixed culture of KUL8, KUL39 and KLB1 were applied for both kinds of wastewater. It was found that better results were observed in palm oil treated with single culture KUL8, KUL39, and KUB1 while mixed culture KUL8 mixed with KLB1 was suitable for use in bakery waste. The three isolates were identified as Acinetobacter sp. (KUL8), Bacillus sp. (KUL39), and Pseudomonas sp. (KLB1). They were all mesophilic strains. Besides, it was found that all three strains also produced amylase and protease which stimulate better waste treatments.
机译:在两组细菌的两个实验中,分别进行了单培养和混合培养的研究,研究了选择产生脂肪酶的细菌对高油脂废水的生物处理。在第一个实验中,用4种细菌分离物处理了烘焙工业废水。结果表明,经过处理后,油脂,COD形式的油脂显着减少。通过单次培养,在7天的处理期间,油脂和COD的去除率分别为73%-88%和81%-99%。分离株KUL8和KUL39显示出更好的活性。然而,与混合培养相比,在单一培养的实验中发现了更好的处理方法。在第二个实验中,在48小时内用6个分离菌处理了棕榈油和烘焙业的废水。所有6种分离物在棕榈油废料中的降解均优于烘焙废料。但是,KUL8和KUL39仍显示出更好的降解活性。两种分离物在棕榈油废水中的脂肪和油脂去除率分别为87.7%和80.6%,在烘焙食品废物中的脂肪和油脂去除率分别为70%和64%。发现化学需氧量的减少为90%-96%。当将KUL8,KUL39和KLB1混合培养用于两种废水时。发现在用单一培养物KUL8,KUL39和KUB1处理的棕榈油中观察到更好的结果,而混合培养物KUL8和KLB1的混合油则适合用于烘焙废料。这三个分离株被鉴定为不动杆菌。 (KUL8),芽孢杆菌属。 (KUL39)和假单胞菌(Pseudomonas sp。) (KLB1)。它们都是嗜温菌株。此外,发现这三株菌株均产生淀粉酶和蛋白酶,刺激了更好的废物处理。

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