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Construction of High Ethanol Fermenting Halotolerant Hybrid by Intergeneric Protoplast Fusion of Saccharomyces cerevisiae and Zygosaccharomyces rouxii

机译:酿酒酵母和鲁氏酵母的原生质体融合融合构建高乙醇发酵耐盐性杂种。

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摘要

Saccharomyces cerevisiae M30, a high ethanol producing flocculent stain, was hybridized with Zygosaccharomyces rouxii TISTR1750, a halotolerant non-flocculent strain, by polyethylene glycol Induced protoplast fusion. The protoplast fusion of these two yeast genera occurred with frequency of 5.89 X 10~-5. Three fusants, namely RM11, Rm37, and RM58, were selected based on their high ethanol fermentation efficiency in glucose broth containing 1.5 and 3/100 sodium chloride. Ethanol fermentation performance in salted medium of the three fusants revealed that in glucose broth containing 1.5, 3, 5 and 7/100 sodium chloride RM11 produced the highest ethanol concentrations and yields when compared to the other two fusants and both parental strains.8
机译:通过聚乙二醇诱导的原生质体融合,将酿酒酵母M30(一种高产乙醇的絮状污渍)与梭状无齿酵母(Zygosaccharomyces rouxii TISTR1750)(一种卤代非絮凝菌株)杂交。这两个酵母属的原生质体融合发生频率为5.89 X 10〜-5。基于它们在含1.5和3/100氯化钠的葡萄糖肉汤中的高乙醇发酵效率,选择了三种融合剂,分别是RM11,Rm37和RM58。三种融合剂在盐培养基中的乙醇发酵性能表明,与其他两种融合剂和两个亲本菌株相比,在含有1.5、3、5和7/100氯化钠的葡萄糖肉汤中,RM11产生的乙醇浓度和产量最高。8

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