Tetraploid Mulberry Induction by Colchicine Treatment through Tissue Culture

Nitsri Sangduen; Ampai Sinpatananon

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Tetraploid Mulberry Induction by Colchicine Treatment through Tissue Culture

机译：秋水仙碱经组织培养处理诱导四倍体桑树

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Tetraploid induction of three mulberry(Morus sp.) varieties Noi, Khunpai and Yai Burirum was obtained from colchicine treatment by soaking aseptic mulberry nodes from tissue culture in 0.02-0.10 /100 colchicine added wit 2/100 dimethy1 sulfoxide(DMSO) solution for 2-5 days. Tetraploid plants were characterized by some indices e.g. thick and dark green leaves, increasing stomatal size and quantity of stomatal chloroplast, decreasing frequency of stomatal per unit area. The chromosome number of root tip cell was 2n= 4x = 56. Tetraploid mulberry attained from this study could be used to cross with diploid plant for further triploid Production.

机译：秋水仙素处理是通过将组织培养中的无菌桑树浸在0.02-0.10 / 100秋水仙碱中加入2/100二甲基亚砜（DMSO）溶液浸泡2天，从秋水仙碱处理中获得的三种桑树（Morus sp。）Noi，Khunpai和Yai Burirum的四倍体诱导。 -5天。四倍体植物的特征在于一些指标，例如叶片厚而深绿色，气孔大小和气孔叶绿体数量增加，单位面积气孔发生频率降低。根尖细胞的染色体数为2n = 4x =56。从该研究中获得的四倍体桑树可用于与二倍体植物杂交以进一步生产三倍体。

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来源
《The Kasetsart Journal》 |1998年第4期|p.424-430|共7页
作者
Nitsri Sangduen; Ampai Sinpatananon;
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原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
mulberry(Mours sp.); colchicine; tetraploid induction; tissue culture;

机译：桑（Mours sp。）;秋水仙碱;四倍体诱导组织培养;

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1. Highly efficient in vitro tetraploid plant production via colchicine treatment using embryogenic suspension cultures in grapevine (Vitis vinifera cv. Mencia) [J] . Acanda Yosvanis, Martinez Oscar, Victoria Gonzalez Maria, Plant Cell, Tissue and Organ Culture: An International Journal on in Vitro Culture of Higher Plants . 2015,第3期

机译：通过秋水仙碱处理使用葡萄中的胚发生悬浮培养物高效生产体外四倍体植物（Vitis vinifera cv。Mencia）
2. Induction of Tetraploid Male Sterile Tagetes erecta by Colchicine Treatment and Its Application for Interspecific Hybridization [J] . HE Yanhong, SUN Yalin, ZHENG Riru, 园艺学报：英文版 . 2016,第005期

机译：秋水仙碱处理诱导四倍体雄性不育万寿菊及其在种间杂交中的应用
3. In vitro induction of tetraploids from immature embryos through colchicine treatments in Clivia miniata Regel [J] . Chong Wang, Jiajun Lei African Journal of Agricultural Research . 2012,第25期

机译：秋水仙碱处理从未成熟胚中体外诱导四倍体
4. Establishment of Embryogenic Cell Suspension Cultures and Doubling the Chromosome Number by Colchicine Treatments in Purple Coneflower [C] . Nilanthi Dahanayake, Chen Xiao-Lu, Zhao Fu-Cheng, 5th International Conference on Bioinformatics and Biomedical Engineering . 2011

机译：紫叶菊秋水仙碱处理建立胚胎细胞悬浮培养物并将染色体数目增加一倍
5. Tetraploid melon from tissue culture and their triploid hybrids. [D] . Adelberg, Jeffrey William. 1993

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6. Induction and Flow Cytometry Identification of Tetraploids from Seed-Derived Explants through Colchicine Treatments in Catharanthus roseus (L.) G. Don [O] . Shi-Hai Xing, Xin-Bo Guo, Quan Wang, 2011

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机译：人工诱导通过血清曲霉治疗和Spathiphyllum Wallisii regel ^ 培养的半吡咯植物^ ^ lsquo;快乐^ ^ rsquo;

Tetraploid Mulberry Induction by Colchicine Treatment through Tissue Culture

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