Production of monoclonal antibodies to flagellar core protein of vibrio parahaemolyticus

Ratchanee Hongprayoon

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Production of monoclonal antibodies to flagellar core protein of vibrio parahaemolyticus

机译：抗副溶血性弧菌鞭毛核心蛋白单克隆抗体的生产

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Polar flagellar core protein was purified from vibrio parahaemolyticus by differential centrifugation and cesium chloride isopycnic centrifugation. Twelve hybridomas secreting monoclonal antibodies against flagellar core protein(MAb-flc) were obtained from fusions. By chance, one purified flagellar core preparation retained flagellar sheath and as a consequence, two hybridomas were detected which secreted anti-sheath antibodies(MAb-fls). MAb-flc and MAb_fls reacted specifically with their corresponding antigens as demonstrated by immunogold labeling.

机译：通过差速离心和氯化铯等温离心从副溶血性弧菌中纯化极性鞭毛核心蛋白。从融合物中获得十二个分泌抗鞭毛核心蛋白（MAb-flc）单克隆抗体的杂交瘤。偶然地，一种纯化的鞭毛核心制剂保留了鞭毛鞘，结果，发现了两种分泌抗鞘层抗体（MAb-fls）的杂交瘤。免疫金标记表明，MAb-flc和MAb_fls与它们相应的抗原发生特异性反应。

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《The Kasetsart Journal》 |1997年第1期|p.106-116|共11页
作者
Ratchanee Hongprayoon;
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原文格式 PDF
正文语种 eng
中图分类自然科学总论;
关键词
vibrio parahaemolyticus; flagellar core protein; flagellar sheath;

机译：副溶血性弧菌鞭毛核心蛋白鞭毛鞘;

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1. Immunomagnetic Separation and Coagglutination of Vibrio parahaemolyticus with Anti-Flagellar Protein Monoclonal Antibody [J] . S. Datta, M. E. Janes, J. G. Simonson Clinical and vaccine immunology: CVI . 2008,第10期

机译：抗纤毛蛋白单克隆抗体对副溶血性弧菌的免疫磁分离和凝集
2. Coagglutination of Vibrio cholerae, Vibrio mimicus, and Vibrio vulnificus with anti-flagellar monoclonal antibody. [J] . J G Simonson, R J Siebeling Journal of Clinical Microbiology . 1988,第10期

机译：霍乱弧菌，模仿弧菌和创伤弧菌与抗鞭毛单克隆抗体的共凝集作用。
3. Production and characterization of a novel monoclonal antibody against Vibrio parahaemolyticus F₀F₁ ATP synthase's delta subunit and its application for rapid identification of the pathogen. [J] . Sakata J, Kawatsu K, Iwasaki T, Journal of Microbiological Methods . 2012,第1期

机译：抗副溶血性弧菌F _{0 F _{1 ATP合酶δ亚基的新型单克隆抗体的制备，鉴定及其在病原菌快速鉴定中的应用。}}
4. Comparison of Manufacture Technologies for RAVI2 Monoclonal Antibody in Production Medium Containing No Animal Derived Proteins [C] . Irene Shackel, Amy Bass, Arno Brewer, General Meeting of European Society of Animal Cell Technology . 2007

机译：没有动物衍生蛋白的生产培养基中的ravi2单克隆抗体制造技术的比较
5. Characterization of orthoreovirus core proteins by sequencing and proteomic analysis of avian reovirus μ proteins and development of α-mammalian reovirus core protein monoclonal antibodies [D] . Noad, Lindsay 2005

机译：通过禽呼肠孤病毒μ蛋白的测序和蛋白质组分析以及α-哺乳动物呼肠孤病毒核心蛋白单克隆抗体的开发来表征正咽病毒核心蛋白
6. Immunomagnetic Separation and Coagglutination of Vibrio parahaemolyticus with Anti-Flagellar Protein Monoclonal Antibody [O] . S. Datta, M. E. Janes, J. G. Simonson 2008

机译：抗纤毛蛋白单克隆抗体的免疫磁分离和凝集性溶血弧菌
7. Immunomagnetic Separation and Coagglutination of Vibrio parahaemolyticus with Anti-Flagellar Protein Monoclonal Antibody▿ [O] . Datta, S., Janes, M. E., Simonson, J. G. 2008

机译：抗鳞茎蛋白单克隆抗体的副溶血性弧菌免疫磁分离和凝集
8. 'Vibrio Cholerae', 'Vibrio Parahaemolyticus', and Other Vibrios Occurence and Distribution in Chesapeake Bay. [R] . Colwell, R. R., Kaper, J., Joseph, S. W. 1977

机译：切里亚克湾的'霍乱弧菌'，'副溶血性弧菌'和其他Vibrios的发生和分布。

Production of monoclonal antibodies to flagellar core protein of vibrio parahaemolyticus

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