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首页> 外文期刊>The Kasetsart Journal >In vitio propagation of ginger (Zingiber officinale roscoe)
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In vitio propagation of ginger (Zingiber officinale roscoe)

机译:姜的断层繁殖(Zingiber officinale Roscoe)

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摘要

Disease free ginger plantlets were produced through tissue culture. The method for surface sterilization was soaking the emerging buds in distilled water containing 0.2/100 tetracycline and 0.2/100 Metalaxyl for one and half hour before treated with 10/100 clorox for 15 minutes followed by 5/100 clorox for 10 minutes. Shoot tips were excised from the buds and planted onto MS medium supplemented with 3 mg/l BA. Eighteen percent of the explants were obtained as clean culture.
机译:通过组织培养产生无病的生姜幼苗。表面灭菌的方法是将萌芽浸入含有0.2 / 100四环素和0.2 / 100甲霜灵的蒸馏水中1个半小时,然后用10/100 clorox处理15分钟,然后用5/100 clorox处理10分钟。从芽上切下茎尖,并种植在补充了3mg / l BA的MS培养基上。 18%的外植体是纯净培养物。

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