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首页> 外文期刊>Tissue Engineering >Effect of Transforming Growth Factor-Beta and Growth Differentiation Factor-5 on Proliferation and Matrix Production by Human Bone Marrow Stromal Cells Cultured on Braided Poly Lactic-Co-Glycolic Acid Scaffolds for Ligament Tissue Engineering
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Effect of Transforming Growth Factor-Beta and Growth Differentiation Factor-5 on Proliferation and Matrix Production by Human Bone Marrow Stromal Cells Cultured on Braided Poly Lactic-Co-Glycolic Acid Scaffolds for Ligament Tissue Engineering

机译：转化生长因子-β和生长分化因子-5对编织韧带-乙醇酸乙醇编织韧带组织工程培养的人骨髓基质细胞增殖和基质产生的影响

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Tissue engineering of ligaments based on biomechanically suitable biomaterials combined with autologous cells may provide a solution for the drawbacks associated with conventional graft material. The aim of the present study was to investigate the contribution of recombinant human transforming growth factor beta 1 (rhTGF-β1) and growth differentiation factor (GDF)-5, known for their role in connective tissue regeneration, to proliferation and matrix production by human bone marrow stromal cells (BMSCs) cultured onto woven, bioabsorbable, 3-dimensional (3D) poly(lactic-co-glycolic acid) scaffolds. Cells were cultured for 12 days in the presence or absence of these growth factors at different concentrations. Human BMSCs attached to the suture material, proliferated, and synthesized extracellular matrix rich in collagen type I and collagen III. No differentiation was demonstrated toward cartilage or bone tissue. The addition of rhTGF-β1 (1–10 ng/mL) and GDF-5 (10–100 ng/mL) increased cell content (p < 0.05), but only TGF-β1 also increased total collagen production (p < 0.05) and collagen production per cell, which is a parameter indicating differentiation. In conclusion, stimulation with rhTGF-β1, and to a lesser extent with GDF-5, can modulate human BMSCs toward collagenous soft tissue when applied to a 3D hybrid construct. The use of growth factors could play an important role in the improvement of ligament tissue engineering.
机译：基于生物力学上合适的生物材料与自体细胞相结合的韧带的组织工程可以为与常规移植材料相关的缺点提供解决方案。本研究的目的是研究重组人转化生长因子β1（rhTGF-β1）和生长分化因子（GDF）-5（其在结缔组织再生中的作用而闻名）对人类增殖和基质产生的贡献骨髓基质细胞（BMSC）培养到编织的，可生物吸收的3维（3D）聚乳酸-乙醇酸支架上。在存在或不存在这些浓度不同的生长因子的情况下，将细胞培养12天。人骨髓间充质干细胞附着在缝合材料上，增殖并合成了富含I型和III型胶原的细胞外基质。没有表现出向软骨或骨组织的分化。添加rhTGF-β1（1–10 ng / mL）和GDF-5（10–100 ng / mL）可增加细胞含量（p <0.05），但只有TGF-β1也可增加胶原蛋白的总产量（p <0.05）和每个细胞的胶原蛋白生成，这是表明分化的参数。总之，当将rhTGF-β1和GDF-5刺激到较小程度时，当将其应用于3D杂交构建体时，可以调节人BMSCs向胶原软组织的方向。生长因子的使用可能在韧带组织工程的改善中起重要作用。

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    《Tissue Engineering》 |2007年第7期|p.1573-1582|共10页
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    J.M.G.Th. Jenner F. van Eijk D.B.F. Saris W.J. Willems W.J.A. Dhert Laura B. Creemers;

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    J.M.G.Th. Jenner, M.D.Department of Orthopedics, University Medical Center, Utrecht, The Netherlands.Department of Orthopedics, OLVG, Amsterdam, The Netherlands.F. van Eijk, M.D.Department of Orthopedics, University Medical Center, Utrecht, The Netherlands.Department of Orthopedics, OLVG, Amsterdam, The Netherlands.D.B.F. Saris, M.D., Ph.D.Department of Orthopedics, University Medical Center, Utrecht, The Netherlands.W.J. Willems, M.D., Ph.D.Department of Orthopedics, OLVG, Amsterdam, The Netherlands.W.J.A. Dhert, M.D., Ph.D.Department of Orthopedics, University Medical Center, Utrecht, The Netherlands.Laura B. Creemers, Ph.D.Department of Orthopedics, University Medical Center, Utrecht, The Netherlands.;

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