• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Toxicological and Environmental Chemistry >APPLICATION OF THE SINGLE-CELL GEL-ELECTROPHORESIS ('COMET') ASSAY TO THE DETECTION OF PRIMARY DNA DAMAGE IN WORKERS OF THE RUBBER INDUSTRY: COMPARISON OF MANUAL AND COMPUTERIZED ANALYSIS
【24h】

APPLICATION OF THE SINGLE-CELL GEL-ELECTROPHORESIS ('COMET') ASSAY TO THE DETECTION OF PRIMARY DNA DAMAGE IN WORKERS OF THE RUBBER INDUSTRY: COMPARISON OF MANUAL AND COMPUTERIZED ANALYSIS

机译：单细胞凝胶电泳（“彗星”分析）在橡胶行业工人DNA损伤检测中的应用：手动和计算机分析的比较

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A recent biochemical technique, the alkaline single-cell microgel-electrophoresis ("comet") assay, allows the evaluation of damage occurred at the DNA level in individual cells associated with exposure to genotoxic compounds. The extent of DNA damage may be directly evaluated by the use of simple visual techniques. However, the development of computerized image analysis sys- tems specifically devoted to this assay has made possible an easier and more detailed measure. In a biological monitoring approach aimed to assess the genotoxic hazard following rubber pro- cessing, peripheral blood leukocytes of l9 male workers from the rubber industry and of age- matched controls living in the same area were analyzed for the presence of DNA damage using the 'comet" assay. In this paper we report results obtained after a re-analysis by a computerized system of photothecrographs previously used for the direct manual measurement of comet length. Considering tail parameters such as tail intensity and tail moment, a statistically significant higher extent of DNA damage was demonstrated in exposed subjects, as compared to controls. On the contrary, visual analysis gave negative results.
机译：最近的一种生物化学技术，即碱性单细胞微凝胶电泳（“彗星”）测定法，可以评估与暴露于遗传毒性化合物有关的单个细胞在DNA水平发生的损伤。 DNA损伤的程度可通过使用简单的视觉技术直接评估。但是，专门用于此测定法的计算机图像分析系统的开发使更简便，更详细的测量成为可能。在一种旨在评估橡胶加工后的遗传毒性危害的生物监测方法中，对橡胶工业的19名男性工人和居住在同一地区的年龄匹配的对照组的外周血白细胞进行了分析，使用“在本文中，我们报告的结果是通过计算机摄影系统重新分析后获得的，该系统以前用于直接手动测量彗星长度。考虑到诸如尾部强度和尾矩之类的尾部参数，具有统计学意义的更高程度与对照组相比，暴露的受试者表现出DNA损伤，相反，视觉分析得出阴性结果。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号