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首页> 外文期刊>Translational Stroke Research >Ventricular Fibrillation-Induced Cardiac Arrest in the Rat as a Model of Global Cerebral Ischemia
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Ventricular Fibrillation-Induced Cardiac Arrest in the Rat as a Model of Global Cerebral Ischemia

机译：大鼠心室纤颤诱发的心脏骤停作为全脑缺血的模型

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摘要

Cardiopulmonary arrest remains as one of the leading causes of death and disability in Western countries. Although ventricular fibrillation (VF) models in rodents mimic the “square wave” type of insult (rapid loss of pulse and pressure) commonly observed in adult humans at the onset of cardiac arrest (CA), they are not popular because of the complicated animal procedure, poor animal survival, and thermal injury. Here, we present a modified, simple, and reliable VF-induced rat model of CA that will be useful in studying the mechanisms of CA-induced delayed neuronal death, as well as the efficacy of neuroprotective drugs. CA was induced in male Sprague Dawley rats using a modified method of von Planta et al. (J Appl Physiol 65:2641–2647, 1988). In brief, VF was induced in anesthetized, paralyzed, and mechanically ventilated rats by an alternating current delivered to the entrance of the superior vena cava into the heart. Resuscitation was initiated by administering a bolus injection of epinephrine and sodium bicarbonate followed by mechanical ventilation and manual chest compressions and countershock with a 10-J direct current. Neurological deficit score was higher in the CA group compared to the sham group during early reperfusion periods, suggesting brain damage. Significant damage in the CA1 hippocampus (21 % normal neurons compared to control animals) was observed following histopathological assessment at 7 days of reperfusion. We propose that this method of VF-induced CA in the rat provides a tool to study the mechanism of CA-induced neuronal death without compromising heart functions.
机译：心肺骤停仍然是西方国家死亡和残疾的主要原因之一。尽管啮齿动物的心室纤颤（VF）模型模仿成年人在心脏骤停（CA）发作时通常观察到的“方波”类型的侮辱（脉搏和压力快速损失），但由于动物复杂，它们并不受欢迎程序，较差的动物存活率和热损伤。在这里，我们提出了一种经过修改，简单且可靠的VF诱导的CA大鼠模型，该模型将有助于研究CA诱导的延迟性神经元死亡的机制以及神经保护药物的功效。使用von Planta等人的改良方法在雄性Sprague Dawley大鼠中诱导CA。 （J Appl Physiol 65：2641-2647，1988）。简而言之，通过输送到上腔静脉入口进入心脏的交流电，在麻醉，瘫痪和机械通气的大鼠中诱发了VF。通过大剂量注射肾上腺素和碳酸氢钠，然后进行机械通气，手动胸部按压和以10-J直流电进行的反击来开始复苏。在早期的再灌注期间，与假手术组相比，CA组的神经功能缺损评分更高，表明脑损伤。在再灌注7天后进行了组织病理学评估，观察到CA1海马区明显受损（与对照动物相比，正常神经元为21％）。我们建议这种在大鼠中由VF诱导的CA的方法提供了一种工具，可以在不损害心脏功能的情况下研究CA诱导的神经元死亡的机制。

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  • 来源
    《Translational Stroke Research》 |2013年第5期|571-578|共8页
  • 作者

    Kunjan R. Dave; David Della-Morte; Isabel Saul; Ricardo Prado; Miguel A. Perez-Pinzon;

  • 作者单位

    Cerebral Vascular Disease Research Laboratories Department of Neurology Leonard M. Miller School of Medicine University of Miami">(1);

    Cerebral Vascular Disease Research Laboratories Department of Neurology Leonard M. Miller School of Medicine University of Miami">(1);

    Department of Advanced Biotechnologies and Bioimaging IRCCS San Raffaele">(3);

    Cerebral Vascular Disease Research Laboratories Department of Neurology Leonard M. Miller School of Medicine University of Miami">(1);

    Cerebral Vascular Disease Research Laboratories Department of Neurology Leonard M. Miller School of Medicine University of Miami">(1);

    Cerebral Vascular Disease Research Laboratories Department of Neurology Leonard M. Miller School of Medicine University of Miami">(1);

    Neuroscience Program Leonard M. Miller School of Medicine University of Miami">(2);

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  • 正文语种 eng
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  • 关键词

    Hippocampus; Global cerebral ischemia; Cardiac arrest; Animal model;

    机译：海马;全脑缺血心脏停搏;动物模型;

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