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Robotic spotting of cDNA and oligonucleotide microarrays

机译:自动发现cDNA和寡核苷酸微阵列

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摘要

DNA microarrays are a uniquely efficient method for simultaneously assessing the expression levels of thousands of genes.Owing to their flexibility and value,mechanically spotted microarrays remain the most popular platform.Here,we review recent technological advances with a focus on spotted arrays.Robotic spotting still poses numerous technical challenges.To reduce artefacts,many laboratories have recently investigated ways of improving the spotting process.We compare alternative options and discuss implications for next-generation systems.Together with modern approaches to data analysis,such developments bring greatly improved reliability to individual microarray experiments.Advancing towards the ultimate goal of delivering calibrated,truly quantitative gene-expression measurements on a genomic scale,microarray technology remains at the forefront of post-genomic systems biology.
机译:DNA微阵列是一种同时评估数千种基因表达水平的独特高效方法。由于其灵活性和价值,机械斑点微阵列仍是最受欢迎的平台。在此,我们将重点关注斑点阵列来综述最近的技术进展。仍然提出了许多技术挑战。为了减少伪像,许多实验室最近研究了改进点样过程的方法。我们比较了替代选择,并讨论了对下一代系统的影响。再加上现代的数据分析方法,这些发展极大地提高了可靠性。微阵列技术始终处于后基因组系统生物学的最前沿,朝着在基因组规模上提供经过校准的,真正定量的基因表达测量的最终目标前进。

著录项

  • 来源
    《Trends in Biotechnology》 |2005年第7期|p.374-379|共6页
  • 作者单位

    Department of Genetics,University of Cambridge,Downing Street,Cambridge CB2 3EH,UK;

    Department of Genetics,University of Cambridge,Downing Street,Cambridge CB2 3EH,UK;

    Inference Group,Cavendish Laboratory,Dept of Physics,University of Cambridge,Madingley Road,Cambridge CB3 OHE,UK;

    Department of Genetics,University of Cambridge,Downing Street,Cambridge CB2 3EH,UK;

    Department of Genetics,University of Cambridge,Downing Street,Cambridge CB2 3EH,UK;

    Department of Genetics,University of Cambridge,Downing Street,Cambridge CB2 3EH,UK;

    Department of Genetics,University of Cambridge,Downing Street,Cambridge CB2 3EH,UK;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 分子生物学;
  • 关键词

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