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首页> 外文期刊>Tropical Animal Health and Production >Epidemiological, bacteriological and molecular studies on caseous lymphadenitis in Sirohi goats of Rajasthan, India
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Epidemiological, bacteriological and molecular studies on caseous lymphadenitis in Sirohi goats of Rajasthan, India

机译：印度拉贾斯坦邦Sirohi山羊干酪性淋巴结炎的流行病学，细菌学和分子学研究

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摘要

Corynebacterium pseudotuberculosis is the causative agent of caseous lymphadenitis (CL), a chronic debilitating disease of goats. In the present study, a total of 575 goats of Sirohi breed on an organized farm situated in the semi-arid tropical region of Rajasthan, India were clinically examined. Pus samples from superficial lymph nodes of 27 (4.7%) adult goats presenting clinical lesions suggestive of CL were collected for bacteriological and molecular analyses. Of these goats, 51.9% yielded C. pseudotuberculosis on the basis of morphological, cultural and biochemical characteristics. A polymerase chain reaction (PCR) assay targeting proline iminopeptidase gene specific to C. pseudotuberculosis was developed that confirmed all 14 bacterial isolates. The specificity of the PCR product was confirmed by sequencing of the 551-bp amplicon in both senses, showing 98–100% homology with published sequences. Thus, overall prevalence rate based on clinical, bacterial culture and PCR assay were found to be 4.7%, 2.4% and 2.4%, respectively. The PCR assay developed in this study was found to be specific and rapid, and could be used for confirmation of CL in goats as an alternative method to generally cumbersome, time-consuming and less reliable conventional methods.
机译：假结核棒杆菌是干酪性淋巴结炎（CL）的病原体，后者是山羊的一种慢性衰弱性疾病。在本研究中，对位于印度拉贾斯坦邦半干旱热带地区的有组织农场中的575只Sirohi山羊进行了临床检查。收集来自27只（4.7％）成年山羊的浅表淋巴结的脓液样本，这些样本表现出提示CL的临床病变，以进行细菌学和分子分析。在这些山羊中，根据形态，文化和生化特征，有51.9％的人生产假结核梭菌。开发了一种针对脯氨酸亚氨基肽酶基因的聚合酶链反应（PCR）测定法，该基因特异于假结核杆菌，确认了所有14种细菌分离株。通过在两个方向上对551 bp的扩增子进行测序，证实了PCR产物的特异性，与已发表的序列显示98-100％的同源性。因此，基于临床，细菌培养和PCR测定的总患病率分别为4.7％，2.4％和2.4％。发现这项研究中开发的PCR检测是特异性和快速的，可用于确认山羊中的CL，作为通常笨重，耗时且可靠性较低的常规方法的替代方法。

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