Genetic evaluation of IS900 partial sequence of Mycobacterium avium subsp. paratuberculosis Brazilian isolates from bovine milk

Isabel Azevedo Carvalho; Victor Oliveira Silva; Pedro Marcus Pereira Vidigal; Abelardo Silva Junior; Maria Aparecida Scatamburlo Moreira

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Genetic evaluation of IS900 partial sequence of Mycobacterium avium subsp. paratuberculosis Brazilian isolates from bovine milk

机译：鸟分枝杆菌亚种IS900部分序列的遗传评估。巴西牛副结核病分离株

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Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis. Insertion sequence IS900 is used for the identification of MAP. The objective of this study was to verify the genetic conservation of IS900 sequences in raw milk samples. To evaluate genetic conservation, 206 quarter milk samples and 16 bulk-tank milk samples were collected. DNA extraction and IS900 PCR were performed in all samples. Six samples amplified the expected fragment. To confirm the identity of the amplified fragments, PCR products were cloned and sequenced. The resulting sequences were compared with other MAP sequences from GenBank, and it was possible to identify eight polymorphic regions and to form five distinct haplotypes. The number of mutations in each haplotype was verified. IS900 sequence is a very well-conserved sequence that could be used as tool for the molecular detection of this agent and epidemiological purposes. The results showed the first genetic analysis on Brazilian isolates of MAP.

机译：鸟分枝杆菌亚种副结核病（MAP）是副结核病的病原体。插入序列IS900用于MAP的识别。这项研究的目的是验证生乳样品中IS900序列的遗传保守性。为了评估遗传保护，收集了206个季度的牛奶样品和16个散装罐式牛奶样品。在所有样品中进行DNA提取和IS900 PCR。六个样品扩增了预期的片段。为了确认扩增片段的身份，将PCR产物克隆并测序。将得到的序列与GenBank的其他MAP序列进行比较，可以鉴定出八个多态性区域并形成五个不同的单倍型。验证了每个单倍型中的突变数。 IS900序列是一个非常保守的序列，可以用作对该剂进行分子检测和流行病学研究的工具。结果显示了对巴西MAP分离株的首次遗传分析。

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《Tropical Animal Health and Production》 |2012年第7期|1331-1334|共4页
作者
Isabel Azevedo Carvalho; Victor Oliveira Silva; Pedro Marcus Pereira Vidigal; Abelardo Silva Junior; Maria Aparecida Scatamburlo Moreira;
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作者单位

Setor de Medicina Veterinária Preventiva e Saúde Pública Departamento de Veterinária Universidade Federal de Viçosa Minas Gerais 36570-000 Brazil;

Setor de Medicina Veterinária Preventiva e Saúde Pública Departamento de Veterinária Universidade Federal de Viçosa Minas Gerais 36570-000 Brazil;

Laboratório de Infectologia Molecular Animal–BIOAGRO–Instituto de Biotecnologia Aplicada à Agropecuária Universidade Federal de Viçosa Minas Gerais 36570-000 Brazil;

Setor de Medicina Veterinária Preventiva e Saúde Pública Departamento de Veterinária Universidade Federal de Viçosa Minas Gerais 36570-000 Brazil;

Setor de Medicina Veterinária Preventiva e Saúde Pública Departamento de Veterinária Universidade Federal de Viçosa Minas Gerais 36570-000 Brazil;

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正文语种 eng
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关键词
Mycobacterium avium subspecies paratuberculosis; Johne’s disease; IS900-PCR; Milk;

机译：禽分枝杆菌亚种副结核病;约翰氏病;IS900-PCR;牛奶;

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1. Genetic evaluation of IS900 partial sequence of Mycobacterium avium subsp paratuberculosis Brazilian isolates from bovine milk [J] . Carvalho Isabel Azevedo, Silva Victor Oliveira, Pereira Vidigal Pedro Marcus, Tropical Animal Health and Production . 2012,第7期

机译：牛分枝杆菌巴西分枝杆菌副菌株IS900 IS900部分序列的遗传评估
2. Mycobacterium avium subsp. Paratuberculosis and M. A. avium Detected by Culture, IS900 and IS901 Highly Sensitive PCR in Bulk Tank Milk from Dairy Herds in the Czech Republic between 2002 and 2004 [J] . Czech Journal of Food Sciences . 2009,第5期

机译：鸟分枝杆菌亚种在2002年至2004年之间，通过文化，IS900和IS901高灵敏度PCR对捷克乳牛群散装罐装牛奶中检出的副结核病和禽链球菌进行了检测
3. Short-Sequence-Repeat Analysis of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium Isolates Collected from Animals Throughout the United States Reveals Both Stability of Loci and Extensive Diversity [J] . N. Beth Harris, Janet B. Payeur, Vivek Kapur, Journal of Clinical Microbiology . 2006,第8期

机译：鸟分枝杆菌亚种的短序列重复分析。副结核病和鸟分枝杆菌亚种。从整个美国的动物中收集的禽分离物揭示了基因座的稳定性和广泛的多样性
4. Detection of Mycobacterium avium subsp. paratuberculosis IS900 in lesions of bovine paratuberculosis by a supersensitive in situ hybridization technique [C] . Tanaka S, Kai T, Yamada N, International Colloquium on Paratuberculosis . 2007

机译：检测分枝杆菌亚空分枝杆菌。 Paratuberculosis is900在原位杂交技术中超敏的牛解霉菌病变
5. Optimization of methods for culturing Mycobacterium avium subsp. paratuberculosis from bovine milk and colostrum and application to samples collected from naturally infected dairy cows. [D] . Bradner, Laura Kaitlan. 2013

机译：鸟分枝杆菌亚种培养方法的优化。牛乳和初乳中的副结核病，并应用于从自然感染的奶牛采集的样品。
6. Short-Sequence-Repeat Analysis of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium Isolates Collected from Animals Throughout the United States Reveals Both Stability of Loci and Extensive Diversity [O] . N. Beth Harris, Janet B. Payeur, Vivek Kapur, 2006

机译：鸟分枝杆菌亚种的短序列重复分析。副结核病和鸟分枝杆菌亚种。从整个美国的动物中收集的禽分离物揭示了基因座的稳定性和广泛的多样性
7. Short-Sequence-Repeat Analysis of Mycobacterium avium subsp. paratuberculosis and Mycobacterium avium subsp. avium Isolates Collected from Animals Throughout the United States Reveals Both Stability of Loci and Extensive Diversity [O] . Harris, N. Beth, Payeur, Janet B., Kapur, Vivek, 2006

机译：鸟分枝杆菌亚种的短序列重复分析。副结核病和鸟分枝杆菌亚种。从整个美国的动物中收集的禽分离物揭示了基因座的稳定性和广泛的多样性

Genetic evaluation of IS900 partial sequence of Mycobacterium avium subsp. paratuberculosis Brazilian isolates from bovine milk

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