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首页> 外文期刊>Tropical Animal Health and Production >Sequence and topological characterization of Toll-like receptor 8 gene of Indian riverine buffalo (Bubalus bubalis)
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Sequence and topological characterization of Toll-like receptor 8 gene of Indian riverine buffalo (Bubalus bubalis)

机译：印度河水牛（Bubalus bubalis）Toll样受体8基因的序列和拓扑表征

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摘要

In this study, buffalo (Bubalus bubalis) Toll-like receptor 8 (TLR8) gene has been characterized by sequence analysis and detecting polymorphism. Complete ORF of buffalo TLR8 gene was amplified using the RNA isolated from spleen tissue, which was found to be 3,102 nucleotides long encoding a 1,033 amino acid protein. Buffalo TLR8 had 10 nucleotide changes as compared to other livestock species resulting in six unique amino acid changes, four of them lying within leucine-rich repeat (LRR) domains. As compared to cattle (Bos indicus and Bos taurus), out of fifteen cysteine residues, fourteen were conserved and Cys at position 521 was replaced by Arg. Nine of the LRR domains had no amino acid change as compared to cattle, whereas LRR-C-terminus had maximum, five amino acid changes. Sequence characterization of 12 riverine and swamp buffaloes revealed presence of four polymorphic nucleotides, two of them were non-synonymous, one synonymous and one site in 3′UTR. PCR-RFLP genotyping of non-synonymous SNP 2758A>G (ILeu920Val) in Toll–interleukin-1 receptor domain of 463 swamp and riverine buffaloes showed a higher frequency of allele A in swamp (95 %) as compared to riverine (9.84 %) buffaloes.
机译：在这项研究中，水牛（Bubalus bubalis）Toll样受体8（TLR8）基因已通过序列分析和检测多态性进行了表征。使用从脾脏组织中分离的RNA扩增了水牛TLR8基因的完整ORF，发现该RNA长3,102个核苷酸，编码1,033个氨基酸。与其他牲畜相比，布法罗TLR8的核苷酸有10个变化，导致六个独特的氨基酸变化，其中四个位于富含亮氨酸的重复（LRR）域中。与牛（印度s和金牛座）相比，在15个半胱氨酸残基中，有14个被保留，521位的Cys被Arg取代。与牛相比，九个LRR结构域没有氨基酸变化，而LRR-C末端最大，有五个氨基酸变化。 12个河豚和沼泽水牛的序列特征表明存在4个多态性核苷酸，其中2个是非同义的，一个同义，一个在3'UTR中的位点。 463沼泽和河水牛的Toll-白介素-1受体域中非同义SNP 2758A> G（ILeu920Val）的PCR-RFLP基因分型显示，与河水（9.84％）相比，沼泽中等位基因A的发生频率更高（95％）水牛。

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