Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed

Jean-Christophe Bambou; Jean-Luc Gourdine; Roxanne Grondin; Nathalie Vachiery; David Renaudeau

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Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed

机译：热挑战对外周血单核细胞活力的影响：热带和温带猪品种的比较

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We evaluated the effect of heat challenge on cell viability, concanavalin A-induced proliferation and heat shock protein (HSPs) mRNA expression in peripheral mononuclear blood cells (PBMC) isolated from Creole (CR) and Large White (LW) pigs. The PBMCs were cultured for 9 h at 37°C before being subjected to heat challenge: (1) at 42°C or 45°C for 2, 4, 6 and 9 h to monitor cell viability;(2) at 45°C for 2 and 9 h followed by stimulation for 24 h at 37°C with concanavalin A to evaluate mitogen-induced proliferation; and (3) at 45°C for 3, 6 and 9 h to measure induction of HSP70.2 and HSP90 mRNA. Cell viability was affected by breed and temperature (P < 0.01), and the viability decrease caused by heat challenge was greater for LW than CR pigs. For mitogen-stimulated PBMCs, incubation at 45°C reduced lymphoblastogenesis equally in both breeds (P < 0.01). Although heat challenge for 3 and 6 h at 45°C induced expression of HSP70.2 and HSP90 mRNA, no breed difference was observed. In conclusion, differences in heat resistance between these two breeds at the whole organism level are reflected at the cellular level. Neither HSP70.2 nor HSP90 mRNA expression levels explain this effect.

机译：我们评估了热挑战对细胞存活率，伴刀豆球蛋白A诱导的增殖和热休克蛋白（HSPs）mRNA表达的影响，这些细胞从克里奥尔（CR）和大白猪（LW）分离出的外周单个核血细胞（PBMC）中。将PBMC在37°C下培养9小时，然后再进行热攻击：（1）在42°C或45°C下进行2、4、6和9小时以监测细胞活力;（2）在45°C下2小时和9小时，然后在37℃用伴刀豆球蛋白A刺激24小时，以评估有丝分裂原诱导的增殖；（3）在45℃下3、6和9小时，以测量HSP70.2和HSP90 mRNA的诱导。细胞存活力受品种和温度的影响（P <0.01），并且与CR猪相比，LW由热激发引起的存活力下降更大。对于有丝分裂原刺激的PBMC，在两个品种中在45°C孵育均能同样降低淋巴母细胞生成（P <0.01）。尽管在45°C下进行3和6小时的热刺激诱导了HSP70.2和HSP90 mRNA的表达，但未观察到品种差异。总之，在整个生物体水平上，这两个品种之间的耐热性差异反映在细胞水平上。 HSP70.2和HSP90 mRNA表达水平均不能解释这种作用。

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《Tropical Animal Health and Production》 |2011年第8期|1535-1541|共7页
作者
Jean-Christophe Bambou; Jean-Luc Gourdine; Roxanne Grondin; Nathalie Vachiery; David Renaudeau;
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INRA UR143 Unité de Recherches Zootechniques Centre INRA Antilles Guyane Domaine de Duclos Prise d’Eau 97170 Petit Bourg Guadeloupe (French West Indies) France;

INRA UR143 Unité de Recherches Zootechniques Centre INRA Antilles Guyane Domaine de Duclos Prise d’Eau 97170 Petit Bourg Guadeloupe (French West Indies) France;

INRA UR143 Unité de Recherches Zootechniques Centre INRA Antilles Guyane Domaine de Duclos Prise d’Eau 97170 Petit Bourg Guadeloupe (French West Indies) France;

UMR 15 CIRAD/INRA Contrôle des maladies animales exotiques et émergentes Domaine de Duclos Prise d’Eau 97170 Petit Bourg Guadeloupe (French West Indies) France;

INRA UR143 Unité de Recherches Zootechniques Centre INRA Antilles Guyane Domaine de Duclos Prise d’Eau 97170 Petit Bourg Guadeloupe (French West Indies) France;

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关键词
Heat challenge; Cell viability; Peripheral blood mononuclear cells; Creole and Large White pigs;

机译：热挑战;细胞活力;外周血单个核细胞;克里奥尔和大型白猪;

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1. Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed. [J] . Bambou J. C., Gourdine J. L., Grondin R., Tropical Animal Health and Production . 2011,第8期

机译：热挑战对外周血单核细胞活力的影响：热带和温带猪品种的比较。
2. Dynamics of cytokine gene expression in peripheral blood mononuclear cells of indigenous and exotic breeds of pigs in India [J] . Animal Science Journal . 2017,第11期

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4. CHANGES IN PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC) AFTER EXPERIMENTAL INFECTION OF PIGS WITH HAEMOPHILUS PARASUIS [C] . A.J. Martin de la Fuente, C.B. Gutierrez Martin, F. Tejerina, Congress ofthe International Pig Veterinary Society . 2008

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Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed

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