Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed.

Bambou J. C.; Gourdine J. L.; Grondin R.; Vachiery N.; Renaudeau D.

首页> 外文期刊>Tropical Animal Health and Production >Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed.

【24h】

Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed.

机译：热挑战对外周血单核细胞活力的影响：热带和温带猪品种的比较。

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

文献代查 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

We evaluated the effect of heat challenge on cell viability, concanavalin A-induced proliferation and heat shock protein (HSPs) mRNA expression in peripheral mononuclear blood cells (PBMC) isolated from Creole (CR) and Large White (LW) pigs. The PBMCs were cultured for 9 h at 37 degrees C before being subjected to heat challenge: (1) at 42 degrees C or 45 degrees C for 2, 4, 6 and 9 h to monitor cell viability;(2) at 45 degrees C for 2 and 9 h followed by stimulation for 24 h at 37 degrees C with concanavalin A to evaluate mitogen-induced proliferation; and (3) at 45 degrees C for 3, 6 and 9 h to measure induction of HSP70.2 and HSP90 mRNA. Cell viability was affected by breed and temperature (P<0.01), and the viability decrease caused by heat challenge was greater for LW than CR pigs. For mitogen-stimulated PBMCs, incubation at 45 degrees C reduced lymphoblastogenesis equally in both breeds (P<0.01). Although heat challenge for 3 and 6 h at 45 degrees C induced expression of HSP70.2 and HSP90 mRNA, no breed difference was observed. In conclusion, differences in heat resistance between these two breeds at the whole organism level are reflected at the cellular level. Neither HSP70.2 nor HSP90 mRNA expression levels explain this effect.

机译：我们评估了热刺激对从克里奥尔（CR）和大白（LW）猪分离的外周单个核血细胞（PBMC）中细胞活力，伴刀豆球蛋白A诱导的增殖和热休克蛋白（HSPs）mRNA表达的影响。将PBMC在37摄氏度下培养9小时，然后再进行热挑战：（1）在42摄氏度或45摄氏度下持续2、4、6和9个小时以监测细胞活力;（2）在45摄氏度下2小时和9小时，然后在37℃用伴刀豆球蛋白A刺激24小时，以评估有丝分裂原诱导的增殖; （3）在45摄氏度下持续3、6和9小时，以测量HSP70.2和HSP90 mRNA的诱导。细胞生存力受品种和温度的影响（ P <0.01），并且热激引起的生存力下降比冷猪大。对于有丝分裂原刺激的PBMC，在两个品种中在45°C孵育均会同样降低淋巴母细胞生成（ P <0.01）。尽管在45℃下3和6小时的热刺激诱导了HSP70.2和HSP90 mRNA的表达，但是没有观察到品种差异。总之，这两个品种在整个生物体水平上的耐热性差异反映在细胞水平上。 HSP70.2和HSP90 mRNA表达水平均不能解释这种作用。

著录项

来源
《Tropical Animal Health and Production》 |2011年第8期|共7页
作者
Bambou J. C.; Gourdine J. L.; Grondin R.; Vachiery N.; Renaudeau D.;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类畜牧、动物医学、狩猎、蚕、蜂;
关键词
animal health; blood cells; breed differences; breeds; concanavalin A; effects; gene expression; haematology; heat resistance; heat shock; incubation; Large White; messenger RNA; pig breeds; shock; stimulation; temperature; tropics; pigs; Sus scrofa; Sus; Suidae; Suiformes; Artiodactyla; ungulates; mammals; vertebrates; Chordata; animals; eukaryotes; animal breed; animal breeds; hematology; hogs; mRNA; swine; tropical countries; tropical zones;

机译：动物健康;血液细胞;品种差异;品种;伴刀豆球蛋白A;作用;基因表达;血液学;耐热性;热休克;温育;大白;信使RNA;猪品种;休克;刺激;温度;热带;猪;猪;苏斯;Suidae;Suiformes;木犀科;有蹄类动物;哺乳动物;脊椎动物;鱼类;动物;真核生物;动物品种;动物品种;血液学;猪;mRNA;猪;热带国家;热带地区;

相似文献

外文文献
中文文献
专利

1. Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed. [J] . Bambou J. C., Gourdine J. L., Grondin R., Tropical Animal Health and Production . 2011,第8期

机译：热挑战对外周血单核细胞活力的影响：热带和温带猪品种的比较。
2. Comparison of Three Isolation Techniques for Human Peripheral Blood Mononuclear Cells: Cell Recovery and Viability, Population Composition, and Cell Functionality [J] . Grievink Hendrika W., Luisman Tarik, Kluft Cornelis, Biopreservation and biobanking . 2016,第5期

机译：人外周血单个核细胞的三种分离技术的比较：细胞回收率和生存力，人口组成和细胞功能
3. Selection of stable reference genes in heat stressed peripheral blood mononuclear cells of tropically adapted Indian cattle and buffaloes [J] . KishoreA., SodhiM., KhateK., Molecular and Cellular Probes: The Location, Diagnosis and Monitoring of Disease by Specific Molecules and Cell Lines . 2013,第3a4期

机译：热带适应性印度牛和水牛热应激外周血单个核细胞中稳定参考基因的选择
4. CHANGES IN PERIPHERAL BLOOD MONONUCLEAR CELLS (PBMC) AFTER EXPERIMENTAL INFECTION OF PIGS WITH HAEMOPHILUS PARASUIS [C] . A.J. Martin de la Fuente, C.B. Gutierrez Martin, F. Tejerina, Congress ofthe International Pig Veterinary Society . 2008

机译：嗜血杆菌猪实验感染后外周血单核细胞（PBMC）的变化
5. Blood sample processing for the study of aging, and characterization of caspasemRNA expression in peripheral blood mononuclear cells. [D] . Lacelle, Chantale. 2003

机译：血液样品处理，用于研究衰老以及外周血单核细胞中caspasemRNA表达的特征。
6. Changes in CD4+ CD8+ CD4+ CD8+ and Immunoglobulin M-Positive Peripheral Blood Mononuclear Cells of Postweaning Multisystemic Wasting Syndrome-Affected Pigs and Age-Matched Uninfected Wasted and Healthy Pigs Correlate with Lesions and Porcine Circovirus Type 2 Load in Lymphoid Tissues [O] . Laila Darwich, Joaquim Segalés, Mariano Domingo, 2002

机译：断奶后多系统衰竭综合症感染的猪和年龄匹配的未感染的废弃健康猪的CD4 +CD8 +CD4 +CD8 +和免疫球蛋白M阳性外周血单个核细胞的变化与淋巴组织中病变和猪圆环病毒2型负荷相关
7. P235Relation between myocardial deformation by three-dimensional speckle tracking analysis , control of the hypertension and functional capacity in patients with systemic hypertensionP236Obstructive sleep apnoea is often under-recognised in patients with acute myocardial infarction, but commonly contributes to left ventricular diastolic dysfunctionP237Intrinsic left ventricular dysfunction in Behcet disease in comparison with systemic disease activity: insights from speckle tracking echocardiographyP238Echocardiography changes during singleton and twin pregnancyP239Proposal of two new echocardiographic parameters for the differentiation of pre-capillary from post-capillary pulmonary hypertensionP240The coincidence of implanted device and number of electrodes with the presence of residual fibrotic tissue after transvenous leads extraction assessed in echocardiographic examP241Hemoglobin is an independent predictor of left ventricular hypertrophy in postmenopausal women but not in premenopausal womenP242Left ventricular longitudinal deformation impairment in patients with psoriasis is linked with immunologic activationP243Impaired diastolic functions and left atrial mechanical functions in patients with vitamin-D deficiencyP244Modification of cardiac and vascular function secondary to insulin resistanceP245Impaired right ventricular function is not related to serum galectin-3 concentration in patients with repaired tetralogy of fallotP246Age-adjusted indices of right ventricular (RV) longitudinal function do not adequately reflect the global RV contraction in children with repaired congenital heart defects and RV volume overloadP247Relationship between fractional flow reserve and dobutamine stress echocardiography in coronary artery diseaseP248A retrospective observational comparative study on the negative predictive value of nuclear testing vs. stress echo in pre-operative assessment for patients undergoing solid organ transplantionP249The detection of viable myocardium by dobutamine stress speckle tracking echocardiography in patients with coronary artery diseaseP250VO2 flattening during exercise in heart failure: evidence for a combined low cardiac output and inefficient O2 extractionP251 The dynamic assessment of alveolar-capillary barrier during exercise-echocardiography in heart failure patients with reduced ejection fractionP252The effect of exercise training on cardiac function during exercise stress echo in patients with type 2 diabetes and diastolic dysfunctionP253Age-related maximal exercise O2 extraction differences in a population of apparently healthy subjects at cardiovascular riskP254Difference in the changes of functional mitral regurgitation between semisupine ergometer and handgrip exerciseP255Correlation of annulus size assessed by echo 2d, 3d and multidetector computed tomography in patients undergoing transcatheter aortic valve implantationP256Mitral annulus dynamics: from normal to extensive mixomatous disease-a three-dimensional transoesophageal studyP257Left atrial appendage closure: an echo point of view of 55 casesP258Effect of catheter-based renal denervation on left ventricular function, mass and (un)twist with two-dimensional speckle tracking echocardiographyP259Associations of microRNAs gene expression in peripheral blood mononuclear cells and left ventricular global longitudinal peak strain in patients with essential hypertension [O] . I. Craciunescu, N. Ngiam, BJ. Sun, 2016

机译：心肌变形之间P235Relation通过三维斑点追踪分析，在患者全身hypertensionP236Obstructive睡眠呼吸暂停的高血压和功能的能力控制是经常根据公认在急性心肌梗死，但通常有助于左室舒张dysfunctionP237Intrinsic左心室功能紊乱中白塞氏病与全身性疾病的活性的比较：用于预毛细管的从植入装置和电极数目的毛细血管后肺hypertensionP240The巧合的分化与存在的两个新的超声心动图参数单和双pregnancyP239Proposal期间从斑点追踪echocardiographyP238Echocardiography变化的见解静脉引线后残留的纤维组织提取评估了超声心动图examP241Hemoglobin是绝经后妇女的左心室肥厚的独立预测因素，但不是在premen opausal womenP242Left心室纵向变形损害牛皮癣患者与免疫联activationP243Impaired舒张功能和左心房机械功能患者的心脏和血管功能继发于胰岛素的维生素-d deficiencyP244Modification resistanceP245Impaired右心室功能是不相关的血清半乳凝素3浓度患者右心室（RV）的纵向功能fallotP246Age调整指标的修复四部曲没有充分反映在冠状动脉diseaseP248A追溯血流储备分数和多巴酚丁胺超声心动图之间修复先天性心脏缺陷和RV容积overloadP247Relationship全球儿童RV收缩在术前评估核试验对应力回声的阴性预测值进行可行myocard的实体器官transplantionP249The检测患者的观察对比研究鎓通过多巴酚丁胺斑点跟踪超声心动图在患者的心脏衰竭锻炼期间冠状动脉diseaseP250VO2压扁：证据为一个组合的低心输出量和低效率的O2 extractionP251在心脏衰竭患者运动超声心动图中肺泡 - 毛细血管屏障的动态评估具有降低的喷射对心脏功能的运动锻炼的运动负荷超声过程中患者fractionP252The影响2型糖尿病患者和表面健康受试者的群体在心血管riskP254Difference在功能性二尖瓣返流的semisupine测力计和手柄之间的变化舒张dysfunctionP253Age相关的最大运动O2提取差异环大小的exerciseP255Correlation由回波2D评估，3d和多排的患者正在接受经导管主动脉瓣implantationP256Mitral环动力学计算机断层：从正常到广泛mixomatous疾病三dimensi Onal地区食道studyP257Left心耳闭合：视在左心室功能，质量和（UN）捻基于导管的肾去神经支配的55 casesP258Effect的在外周血单核细胞和左侧的微小RNA基因表达的二维散斑跟踪echocardiographyP259Associations回波点心室全球纵向应变峰值在原发性高血压患者

Effect of heat challenge on peripheral blood mononuclear cell viability: comparison of a tropical and temperate pig breed.

摘要

著录项

相似文献

相关主题

期刊订阅