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首页> 外文期刊>Tropical Animal Health and Production >Isolation and identification of virulent peste des petits ruminants viruses from PPR outbreaks in India
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Isolation and identification of virulent peste des petits ruminants viruses from PPR outbreaks in India

机译：印度PPR爆发的强毒小反刍动物病毒的分离与鉴定

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In this study, three outbreaks of peste des petits ruminants (PPR) in goats and sheep flocks with high morbidity and considerable mortality were recorded at Jhansi and Revati in Uttar Pradesh and Bhopal in Madhya Pradesh, India during 2003–2006. Clinical samples were collected from the affected flocks for laboratory investigation. The PPR virus (PPRV) antigenucleic acid in the infected tissues/swab materials was demonstrated by using sandwich enzyme-linked immunosorbent assay (ELISA) and reverse transcription–polymerase chain reaction techniques, and the antibody to PPRV in serum samples was detected by competitive ELISA. The causative agent of the outbreaks, PPRV, was successfully isolated in Vero cells at first passage itself, and its identity was confirmed. The isolated PPR viruses belong to lineage IV based on phylogenetic analysis of partial fusion gene sequences and are closely related to other Asian or Indian PPRV isolates/strains.
机译：在这项研究中，在印度北方邦的Jhansi和Revati以及印度中央邦的博帕尔，在2003-2006年期间，在高发病率和高死亡率的山羊和绵羊群中记录了三起小反刍兽疫（PPR）暴发。从受影响的鸡群中收集临床样品进行实验室研究。通过夹心酶联免疫吸附测定（ELISA）和逆转录聚合酶链反应技术证明了感染的组织/拭子材料中的PPR病毒（PPRV）抗原/核酸，并通过检测血清中的PPRV抗体竞争ELISA。暴发的病原体PPRV在第一次传代时就成功地在Vero细胞中分离出来，并确认了其身份。根据部分融合基因序列的系统发育分析，分离出的PPR病毒属于IV谱系，并且与其他亚洲或印度PPRV分离株/菌株密切相关。

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