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MULTIPLE ANTIGENIC PEPTIDE ASSAY FOR DETECTION OF PESTE DES PETITS RUMINANTS (PPR) VIRUS SPECIFIC ANTIBODIES
MULTIPLE ANTIGENIC PEPTIDE ASSAY FOR DETECTION OF PESTE DES PETITS RUMINANTS (PPR) VIRUS SPECIFIC ANTIBODIES
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机译：多种抗原肽测定法检测瘟疫病原菌（PPR）病毒特异性抗体。
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摘要
The invention relates to synthetic multiple antigenic peptides (MAPs) mixotope reagent for detecting antibodies to peste des petits ruminants (PPR) virus in the test sera. The invention also relates to a method for detecting and quantitating antibodies to PPR virus in an indirect ELISA, wherein, the MAP mixotope reagent is used as coating antigen. The assay using MAP antigen specifically detects antibodies to PPR virus and shows no cross reactivity with antigenically related rinderpest virus antibodies. The novel diagnostic method using MAP antigen has its application in sero-monitoring of PPR in target population. The MAP assay can also be suitably used for quantitative assessment of humoral immune response in the vaccinated animals. Also, the assay can suitably be sued for diagnosis of PPR in naturally infected animals if paired sera samples are available. In experimental analysis, the MAP-ELISA detected antibodies to PPR virus with sensitivity of 98.3% and specificity 97.5%. The MAP antigen developed in present invention is safe, homogenous, non infectious, non-hazardous and stable at room temperature, therefore, it is aptly suited for serological surveys in different geographical and climatic situations.
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