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Novel, Rapid Assay for Detection and Differentiation of Serotype- Specific Antibodies to Venezuelan Equine Encephalitis Complex Alphaviruses

机译：用于检测和鉴别委内瑞拉马脑炎复合物甲病毒血清型特异性抗体的新型快速检测方法

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An epitope-blocking enzyme-linked immunosorbent assay was developed for the rapid differentiation of serologic responses to enzootic variety IE and ID versus epizootic variety IAB and IC strains of Venezuelan equine encephalitis (VEE) virus. Two monoclonal antibodies that differentially recognize epizootic versus enzootic VEE virus epitopes were used to measure the serotype-specific blocking abilities of antibodies in sera of naturally infected humans, equines, and bovines, as well as in experimentally infected equines. The assay is simple, species-independent, rapid, and sensitive, and will improve surveillance for VEE emergence. It could also be used to determine the epidemic potential of a VEE virus following an intentional introduction for bioterrorism.

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作者
Wang, E. ; Paessler, S. ; Smith, D. R. ; Coffey, L. L. ; Kang, W.;
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年度 2005
页码 1-7
总页数 7
原文格式 PDF
正文语种 eng
中图分类工业技术;
关键词
Venezuelan equine encephalomyelitis virus; Microbiological tests; Reprints; Antibodies; Bioassay; Epizootic;

机译：委内瑞拉马脑脊髓炎病毒;微生物学试验;重印;抗体;生物测定;流行病学;

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专利

1. A NOVEL, RAPID ASSAY FOR DETECTION AND DIFFERENTIATION OF SEROTYPE-SPECIFIC ANTIBODIES TO VENEZUELAN EQUINE ENCEPHALITIS COMPLEX ALPHAVIRUSES. [J] . Weaver SC The American Journal of Tropical Medicine and Hygiene . 2005,第6期

机译：新型快速检测方法，用于检测和区分委内瑞拉马匹脑炎复杂脑炎的血清型特异性抗体。
2. Pseudotyped viruses permit rapid detection of neutralizing antibodies in human and equine serum against Venezuelan equine encephalitis virus. [J] . Kolokoltsov AA, Wang E, Colpitts TM, The American Journal of Tropical Medicine and Hygiene . 2006,第4期

机译：伪型病毒可以快速检测人和马血清中的抗委内瑞拉马脑炎病毒的中和抗体。
3. Development of immunofiltration assay by light addressable potentiometric sensor with genetically biotinylated recombinant antibody for rapid identification of Venezuelan equine encephalitis virus. [J] . Hu WG, Thompson HG, Alvi AZ, Journal of Immunological Methods . 2004,第1a2期

机译：通过光寻址电位传感器和遗传生物素化重组抗体开发免疫过滤测定法，以快速鉴定委内瑞拉马脑炎病毒。
4. Comparative Epidemiologies of Eastern Equine Encephalitis Virus, Western Equine Encephalitis Virus, Venezuelan Equine Encephalitis Virus, and West Nile Virus [C] . Charles H. Calisher Annual Meeting of the American Society for Veterinary Clinical Pathology . 2004

机译：东部大雄脑炎病毒，西马脑炎病毒，委内瑞拉脑脑炎病毒和西尼罗河病毒的比较流行病学
5. Characterization of the Escherichia coli exopolyphosphatase and production of antibodies against the purified recombinant E3 protein of Venezuelan Equine Encephalitis virus. [D] . Ghosh, Anita. 2010

机译：大肠杆菌外多磷酸酶的表征和针对纯化的委内瑞拉马脑炎病毒的重组E3蛋白的抗体的生产。
6. Monoclonal antibodies capable of distinguishing epizootic from enzootic varieties of subtype 1 Venezuelan equine encephalitis viruses in a rapid indirect immunofluorescence assay. [O] . J T Roehrig, R A Bolin 1997

机译：能够在快速间接免疫荧光测定中区分亚型1委内瑞拉马脑炎病毒的流行变种与流行变种的单克隆抗体。
7. Comparison of Dissociation-Enhanced Lanthanide Fluorescent Immunoassays to Enzyme-Linked Immunosorbent Assays for Detection of Staphylococcal Enterotoxin B, Yersinia pestis-Specific F1 Antigen, and Venezuelan Equine Encephalitis Virus [O] . Smith, Darci R., Rossi, Cynthia A., Kijek, Todd M., 2001

机译：解离增强镧系荧光免疫分析法与酶联免疫吸附法检测葡萄球菌肠毒素B，鼠疫耶尔森氏菌特异性F1抗原和委内瑞拉马脑炎病毒的比较

Novel, Rapid Assay for Detection and Differentiation of Serotype- Specific Antibodies to Venezuelan Equine Encephalitis Complex Alphaviruses

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