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Predatory activity of the nematophagous fungus Duddingtonia flagrans on horse cyathostomin infective larvae

机译:线虫真菌杜氏鞭毛虫对马屎藻鞘蛋白感染性幼虫的捕食活性

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This work was performed to determine the predatory capacity in vitro of the nematophagous fungus Duddingtonia flagrans (isolate AC001) on cyathostomin infective larvae of horse (L3). The experimental assay was carried out on plates with 2% water-agar (2% WA). In the treated group, each plate contained 1.000 L3 and 1.000 conidia of the fungus. The control group without fungus only contained 1.000 L3 in the plates. Ten random fields (4 mm diameter) were examined per plate of treated and control groups, every 24 h for seven days under an optical microscope (10× and 40× objective lens) for non-predated L3 counts. After 7 days, the non-predated L3 were recovered from the Petri dishes using the Baermann method. The interaction there was a significant reduction (p < 0.01) of 93.64% in the cyathostomin L3 recovered. The results showed that the D. flagrans is a potential candidate to the biological control of horse cyathostomin L3.
机译:进行这项工作来确定线虫真菌鞭毛杜氏鞭毛虫(分离物AC001)对马Cyathostomin感染幼虫(L 3 )幼虫的体外捕食能力。实验测定是在装有2%水琼脂(2%WA)的板上进行的。在治疗组中,每个平板含有1.000 L 3 和1.000真菌分生孢子。不含真菌的对照组的平板中仅含有1.000 L 3 。在未使用L 3 的光学显微镜(10倍和40倍物镜)下,每24小时对每组治疗和对照组的板片检查10个随机视野(直径4 mm),持续7天。计数。 7天后,使用Baermann方法从培养皿中回收未捕食的L 3 。所回收的c鞘蛋白L 3 的相互作用显着降低(p <0.01)93.64%。结果表明,鞭毛D.鞭毛是马Cyathostomin L 3 生物防治的潜在候选者。

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