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Predatory activity of the nematophagous fungus Duddingtonia flagrans on horse cyathostomin infective larvae

机译:线虫真菌杜氏鞭毛对马屎藻鞘蛋白感染性幼虫的捕食活性

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This work was performed to determine the predatory capacity in vitro of the nematophagous fungus Duddingtonia flagrans (isolate AC001) on cyathostomin infective larvae of horse (L). The experimental assay was carried out on plates with 2% water-agar (2% WA). In the treated group, each plate contained 1.000 L and 1.000 conidia of the fungus. The control group without fungus only contained 1.000 L in the plates. Ten random fields (4 mm diameter) were examined per plate of treated and control groups, every 24 h for seven days under an optical microscope (10c and 40c objective lens) for non-predated L counts. After 7 days, the non-predated L were recovered from the Petri dishes using the Baermann method. The interaction there was a significant reduction (p<0.01) of 93.64% in the cyathostomin L recovered. The results showed that the D. flagrans is a potential candidate to the biological control of horse cyathostomin L.
机译:进行这项工作来确定线虫真菌杜氏鞭毛鞭毛虫(分离物AC001)对马(C)的藻鞘蛋白感染幼虫的体外捕食能力。实验测定是在装有2%水琼脂(2%WA)的平板上进行的。在治疗组中,每个平板含有1.000 L和1.000的真菌分生孢子。不含真菌的对照组的平板中仅含有1.000L。在光学显微镜(10c和40c物镜)下,每24小时检查每组治疗和对照组的十个随机视野(直径4 mm),以观察未掠夺的L计数。 7天后,使用Baermann方法从培养皿中回收未捕食的L。所回收的氰菊酯L中的相互作用显着降低(p <0.01)93.64%。结果表明,D.flagrans是潜在的候选者,可以对马连翘的生物进行控制。

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