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首页> 外文期刊>Tropical Animal Health and Production >Utilization of protein-A in immuno-histochemical techniques for detection of Peste des Petits Ruminants (PPR) virus antigens in tissues of experimentally infected goats
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Utilization of protein-A in immuno-histochemical techniques for detection of Peste des Petits Ruminants (PPR) virus antigens in tissues of experimentally infected goats

机译：蛋白质A在免疫组化技术中用于检测实验感染山羊组​​织中的小反刍动物（PPR）病毒抗原的方法

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摘要

This paper constitutes the first record of utilizing the S. aureus protein-A (PA), conjugated to peroxidase enzyme, for the detection of the Peste des Petits Ruminants (PPR) virus antigens in tissues of experimentally infected goats. The goats were experimentally infected with a virulent PPR virus, which was previously isolated from a severe natural disease outbreak in gazelles, during 2002 in Saudi Arabia. The technique is rapid, and has the superiority over the peroxidase –anti-peroxidase (PAP) test in that, inactivation of the indigenous peroxidase in the tissues is not required and that it can be used against a wide range of animal species. An advantage over the other immunolabelled conjugates is that PA attaches specifically to the crystalizable fraction (Fc) of the IgG molecule, thus allowing the antigen binding fraction (Fab) of the molecule, free to interact specifically with the antigen. So, it doesn't actually compete with the antigen for the Fab portion of the IgG molecule. In the present study, PA conjugate detected the PPR virus antigens in various tissues of the experimentally infected goats.
机译：这篇论文构成了利用与过氧化物酶偶联的金黄色葡萄球菌A蛋白（PA）来检测实验感染山羊组​​织中小反刍动物（PPR）病毒抗原的第一笔记录。山羊实验性感染了强毒的PPR病毒，该病毒先前是在2002年沙特阿拉伯从瞪羚的一次严重自然疾病暴发中分离出来的。该技术是快速的，并且相对于过氧化物酶-抗过氧化物酶（PAP）测试具有优越性，因为它不需要灭活组织中的固有过氧化物酶，并且可以用于多种动物。与其他免疫标记的偶联物相比，优点是PA特异性附着于IgG分子的可结晶级分（Fc），从而使分子的抗原结合级分（Fab）自由与抗原特异性相互作用。因此，它实际上并未与抗原竞争IgG分子的Fab部分。在本研究中，PA结合物在实验感染山羊的各种组织中检测到PPR病毒抗原。

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