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首页> 外文期刊>Tropical Animal Health and Production >Identification of Pasteurella multocida isolates of ruminant origin using polymerase chain reaction and their antibiogram study
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Identification of Pasteurella multocida isolates of ruminant origin using polymerase chain reaction and their antibiogram study

机译：应用聚合酶链反应鉴定反刍动物多杀性巴斯德氏菌菌株及其抗菌谱研究

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摘要

A total of 100 isolates of Pasteurella multocida from various ruminant species (cattle, buffalo and sheep) belonging to different parts of country were identified using Pasteurella multocida-PCR (PM-PCR) and capsular PCR assays. PM-PCR revealed an amplicon of ∼460 bp in all the isolates tested. As regards capsular PCR, 36 of 38 cattle isolates and 30 of 34 buffalo isolates were found to belong to capsular serogroup B whereas rest of the cattle and buffalo isolates belonged to serogroup A of P. multocida. In case of sheep, a total of 26 out of 28 isolates were positive for serogroup A specific PCR while remaining 2 amplified a PCR product specific for serogroup F of P. multocida. All the isolates were subjected to antibiotic sensitivity testing using 17 different antibiotics. Enrofloxacin was found to be most potent antibiotic as it was effective against 94% of the isolates followed by ofloxacin (93%), chloramphenicol (93%), doxycycline (89%), tetracycline (86%) and ciprofloxacin (84%). Vancomycin, bacitracin and sulfadiazine were ineffective against P. multocida isolates showing 84%, 75% and 82% resistance, respectively. Further, the antibiogram also revealed the development of resistance against multiple drugs among various isolates of the organism.
机译：使用多杀巴斯德氏菌-PCR（PM-PCR）和荚膜PCR试验鉴定出来自该国不同地区的各种反刍动物物种（牛，水牛和绵羊）的100株多杀巴斯德氏菌。 PM-PCR在所有测试的分离物中均显示约460 bp的扩增子。关于荚膜PCR，发现38个牛分离株中的36个和34个水牛分离株中的30个属于荚膜血清群B，而其余牛和水牛分离株则属于多杀青霉菌的血清群A。以绵羊为例，在28个分离株中，共有26个对A血清群特异性PCR呈阳性，而其余2个扩增了对P. multocida血清群F特异性的PCR产物。所有分离物均使用17种不同的抗生素进行了抗生素敏感性测试。恩诺沙星是最有效的抗生素，因为它对94％的分离株有效，其次是氧氟沙星（93％），氯霉素（93％），强力霉素（89％），四环素（86％）和环丙沙星（84％）。万古霉素，杆菌肽和磺胺嘧啶对多杀性疟原虫分离株无效，分别显示出84％，75％和82％的耐药性。此外，抗菌谱还揭示了该生物的各种分离物中对多种药物的耐药性的发展。

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