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首页> 外文学位 >Comprehensive genetic analysis of an avian isolate of Pasteurella multocida.
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Comprehensive genetic analysis of an avian isolate of Pasteurella multocida.

机译：穆氏巴斯德氏菌禽分离株的综合遗传分析。

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摘要

Pasteurella multocida is the etiologic agent of a variety of economically significant diseases including fowl cholera in birds, hemorrhagic septicemia in cattle, atrophic rhinitis in swine, and the most common source of infection in man due to dog and cat bites. The global distribution, severity of diseases caused, and the wide variety of livestock affected by P. multocida account for considerable economic losses due to this pathogen worldwide. Current research has provided substantial information regarding P. multocida growth requirements, colony appearance, and biochemical characteristics, however, little is known regarding the molecular basis for P. multocida's pathogenicity. In order to address these issues, we tested the following hypotheses: (i) the genome sequence of P. multocida will aid in the identification of putative genes involved in P. multocida colonization and pathogenicity; and (ii) P. multocida contains sets of metabolic/virulence gene networks that respond to stress.; In order to create a foundation for future studies for a better molecular understanding of P. multocida, the genome of an avian isolate of P. multocida was sequenced. This allowed for identification of all potential genes, intergenic regions, and chromosome organization. Of importance were the identification of putative genes unique to P. multocida, a large set of hypothetical genes with no known function, as well as those putative genes involved in cellular metabolism, transport, and virulence.; In order to elucidate P. multocida's response to extracellular stress, DNA microarray technology was applied to determine the genome-wide transcriptional profiles of P. multocida in response to several environmental stressors: heat shock, acid stress, oxidative stress, and sodium stress. The global patterns of gene expression in P. multocida in response to individual and multiple stressors (the general stress response) were analyzed, and provided insight into the mechanisms by which P. multocida can adapt to stress.
机译：多杀巴斯德氏菌是多种经济上重要疾病的病原体，包括禽类霍乱，牛出血性败血病，猪萎缩性鼻炎以及人为狗和猫引起的人类最常见感染源咬。 Multocida 所影响的全球分布，致病的严重性和各种各样的牲畜造成了这种病原体在世界范围内造成的巨大经济损失。当前的研究提供了有关 P的大量信息。穆尔他达菌的生长要求，菌落外观和生化特性，但是关于<斜体> P的分子基础知之甚少。多杀菌的致病性。为了解决这些问题，我们测试了以下假设：（i） P的基因组序列。多杀将有助于鉴定与 P有关的推定基因。多杀菌的定植和致病性； （ii）<斜体> P。穆尔他达科（italtic）包含一组可应对压力的代谢/毒力基因网络。为了为将来的研究奠定基础，以更好地理解 P。 mitalocida ， P禽分离株的基因组。对多杀菌进行了测序。这样可以鉴定所有潜在的基因，基因间区域和染色体组织。重要的是鉴定 P特有的推定基因。 multocida ，大量未知功能的假定基因，以及与细胞代谢，转运和毒力有关的推定基因。为了阐明 P。 multocida 对细胞外应激的反应，使用DNA芯片技术确定 P的全基因组转录谱。应对多种环境胁迫：热激，酸胁迫，氧化胁迫和钠胁迫。 P中基因表达的整体模式。分析了多杀性疟原虫对单个和多个应激源的响应（一般性应激反应），并为多杀性疟原虫适应压力的机理提供了见解。

著录项

  • 作者

    May, Barbara Jean.;

  • 作者单位

    University of Minnesota.;

  • 授予单位 University of Minnesota.;
  • 学科 Biology Microbiology.; Biology Genetics.
  • 学位 Ph.D.
  • 年度 2002
  • 页码 p.3143
  • 总页数 287
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类 微生物学;
  • 关键词

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