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首页> 外文期刊>Slovenian Veterinary Research >Study on immune efficacy of genomic expression library vaccines against avian Pasteurella multocida.
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Study on immune efficacy of genomic expression library vaccines against avian Pasteurella multocida.

机译：基因组表达文库疫苗对禽多杀性巴氏杆菌的免疫功效研究。

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摘要

Random DNA fragments (500-3000 bp) were obtained from the genome of avian Pasteurella multocida CVCC474 using the restriction enzyme Sau3AI and cloned into the BamHI site of the eukaryotic expression vector pcDNA3.1(+) to construct a genomic expression library. The library was subdivided into five clone pools and recombinant plasmids were extracted from each pool. Balb/c mice were allocated to groups 1-5 and vaccinated with these recombinant plasmids, whereas mice injected with pcDNA3.1(+) and PBS were used as negative controls. The immune responses were evaluated based on the serum antibody level, a lymphocyte proliferation assay, and the IFN- gamma level. The protective efficacy after challenge with the virulent avian Pasteurella multocida strain CVCC474 was evaluated based on the relative protection rates. A significant increase in the serum antibody levels was observed in mice in group 1. The lymphocyte proliferation (SI value) and IFN- gamma levels were also higher in mice immunized with the group 1 vaccine compared with those vaccinated with group 2-5 vaccines (p<0.05) and control groups (p<0.01). Furthermore, group 1 vaccine provided better protection than other vaccines after challenge with avian Pasteurella multocida strain CVCC474. The results indicated that construction of a genomic expression vaccine library is a promising approach for the prevention of avian pasteurellosis.
机译：使用限制性内切酶Sau3AI从禽多杀巴斯德氏菌CVCC474的基因组中获得随机DNA片段（500-3000 bp），并将其克隆到真核表达载体pcDNA3.1（+）的BamHI位点中，以构建基因组表达文库。该文库分为五个克隆库，并从每个库中提取重组质粒。将Balb / c小鼠分配至1-5组，并用这些重组质粒接种，而将注射pcDNA3.1（+）和PBS的小鼠用作阴性对照。基于血清抗体水平，淋巴细胞增殖测定和IFN-γ水平评估免疫应答。基于相对保护率，评价了强毒性禽多杀巴斯德氏菌菌株CVCC474攻击后的保护功效。在第1组的小鼠中观察到血清抗体水平显着增加。与用2-5组疫苗接种的小鼠相比，用1组疫苗免疫的小鼠的淋巴细胞增殖（SI值）和IFN-γ水平也更高（ p <0.05）和对照组（p <0.01）。此外，在感染多杀性巴氏杆菌CVCC474菌株后，第1组疫苗提供了比其他疫苗更好的保护作用。结果表明，构建基因表达疫苗文库是预防禽巴氏杆菌病的一种有前途的方法。

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